Sequences from confirmation amplicon


Confirmation sequences for line 685G11 and hit At5g23260 are shown.
Line ID 685G11
Locus At5g23260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:7836804
Primers used for PCR 25pj - 8474, show primer details
   
Sequence Name 37-K115005-0022-685-G11C-25pj
GenBank Accession LN502722 [GenBank]
Sequencing primer gene specific primer, 25pj: 5'-TTTCACACACACACACACTTTTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K115005-0022-685-G11C-25pj
GATTCAGAATCACAACATCCTTGAAATGAATATTGTAATTAACTAATTCTAGGGTTTTCA
GATTCTTGTTTTTTTTGGTTAGTTAATTAGGGTTTCTTTTAAGTTGATCTAGCTTTCATA
TACACTTGAATCAGGAGGAAGAGGGAGCCACTCATAAGAGGAAGAAGAGAGAGATGGGTA
GAGGGAAGATAGAGATAAAGAAGATAGAGAATCAGACGGCGAGGCAAGTGACCTTCTCCA
AGAGAAGAACTGGTCTTATAAAGAAGACTCGTGAGCTCTCTATTCTCTGTGACGCTCACA
TCGGTCTCATCGTCTTCTCAGCCACCGGAAAGCTTTCCGAGTTCTGCTCCGAACAGAACA
GGTTAACTTCTTCTTCTTTCTTGAATTAGGGTTTCAGAGATCTATACATACATGTGTATG
TATCCATCTAATTAATGAGGAAATGAGCTAGATATATGACTCGTGCTTGAGAACAGACAG
TTTTCATCATTAGTTTCTGAAGTAGCAGGTGTCATGAGTCGTTTTTTGGATTTTCAATTG
TAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATG
GAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACG
GTTATT
   
Sequence Name 05-K115004-0022-685-G11C-8409
GenBank Accession LN488467 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K115004-0022-685-G11C-8409
ATCGGAAGNAGCCATTTCNATTGAAAATCCAAAAAACGACTCATGACACCTGCTACTTCA
GAAACTAATGATGAAAACTGTCTGTTCTCAAGCACGAGTCATATATCTAGCTCATTTCCT
CATTAATTAGATGGATACATACACATGTATGTATAGATCTCTGAAACCCTAATTCAAGAA
AGAAGAAGAAGTTAACCTGTTCTGTTCGGAGCAGAACTCGGAAAGCTTTCCGGTGGCTGA
GAAGACGATGAGACCGATGTGAGCGTCACAGAGAATAGAGAGCTCACGAGTCTTCTTTAT
AAGACCAGTTCTTCTCTTGGAGAAGGTCACTTGCCTCGCCGTCTGATTCTCTATCTTCTT
TATCTCTATCTTCCCTCTACCCATCTCTCTCTTCTTCCTCTTATGAGTGGCTCCCTCTTC
CTCCTGATTCAAGTGTATATGAAAGCTAGATCAACTTAAAAGAAACCCTAATTAACTAAC
CAAAAAAAACAAGAATCTGAAAACCCTAGAATTAGTTAATTACAATATTCATTTCAAGGA
TGTTGTTGATTCTTGAATCTTGATGTGTGAATTAGAGAGGAGATAGAGAGAGAAAAAGTG
TGTGGTGGTGTGAAA

Last Updated on 06.12.2018 16:11