Genotyping and primer details

Genotyping and primer details for 685G11 - At5g23260
Line ID 685G11
Locus At5g23260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:7836804 of line 685G11 Chr5:7836804
Insertion allele amplicon 8474 - 25pj
Locus specific primer 25pj: 5'-TTTCACACACACACACACTTTTTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:7836211
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11