Sequences from confirmation amplicon


Confirmation sequences for line 686D04 and hit At1g78460 are shown.
Line ID 686D04
Locus At1g78460 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:29519113
Primers used for PCR AU12 - 8474, show primer details
   
Sequence Name 32-K106287-0022-686-D04C-AU12
GenBank Accession LN500594 [GenBank]
Sequencing primer gene specific primer, AU12: 5'-TCGGTTCTAAACCACCTCTTCTAA-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K106287-0022-686-D04C-AU12
TCAATCGTGAGGGCGTCATTAACATGATCTACATGTTAAACGGAACCATGATCTCATTAA
TCACCCTAGCCGTGGTATGAGATGGCGGGTTATAAACAGCGAGAAAATACGCCGGCAACT
TTCCTTTGCTCTTCTCGATGGGCAAAATCCAGTTAGAATCCCGGAGGCTTTCCATCAGAG
CCGCAGCTTCGTCCTTGGCTACATTGTTCGACACGTAGCCACCGATCTGTCTCACGGCTA
CGTAAGTTGGTTTCGTAGACCGAACGTGGAGATCATCGGCCTGAGGTGGGTTCTGTTGGT
TCTTCTTCGGGAGGTAGAGTGAGACCGTGTAGACTGATCTTCCAGGCGTTGCTTGTGCGA
TCACCGGAGCTGTCATGTTCATCTTCACGTTGCTCTTGTTATCTCCTTCTATGTACCTAT
TTAATCTATAAACCCAAAAACGTAGTCAACTTCAGTTTATAATATAAAGAAACCGAAAGA
ACACCAAGTCTTATATATTCGATTTATTCATTTACCGTCGGAAACCGGTTTTGGTGGCTT
GAGTCATGGATAGGGAGGGGATAGGACTAGTAGATATCCATAGGGCAGCGTCGTATGGCT
TCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 32-K106286-0022-686-D04C-8409
GenBank Accession LN500593 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K106286-0022-686-D04C-8409
GCCATACGACGCTGCCCTATGGATATCTACTAGTCCTATCCCCTCCCTATCCATGACTCA
AGCCACCAAAACCGGTTTCCGACGGTAAATGAATAAATCGAATATATAAGACTTGGTGTT
CTTTCGGTTTCTTTATATTATAAACTGAAGTTGACTACGTTTTTGGGTTTATAGATTAAA
TAGGTACATAGAAGGAGATAACAAGAGCAACGTGAAGATGAACATGACAGCTCCGGTGAT
CGCACAAGCAACGCCTGGAAGATCAGTCTACACGGTCTCACTCTACCTCCCGAAGAAGAA
CCAACAGAACCCACCTCAGGCCGATGATCTCCACGTTCGGTCTACGAAACCAACTTACGT
AGCCGTGAGACAGATCGGTGGCTACGTGTCGAACAATGTAGCCAAGGACGAAGCTGCGGC
TCTGATGGAAAGCCTCCGGGATTCTAACTGGATTTTGCCCATCGAGAAGAGCAAAGGAAA
GTTGCCGGCGTATTTTCTCGCTGTTTATAACCCGCCATCTCATACCACGGCTAGGGTGAT
TAATGAGATCATGGTTCCGTTTAACATGTAGATCATGTTAATGACGCCCTCACGATTTGA
TTGTTACGTAGCANTTGGATTAGAAGAGGT

Last Updated on 06.12.2018 16:11