Sequences from confirmation amplicon


Confirmation sequences for line 686D07 and hit At2g30140 are shown.
Line ID 686D07
Locus At2g30140 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:12872317
Primers used for PCR MG10 - 8474, show primer details
   
Sequence Name 15-K031455-022-686-D07C-MG10
GenBank Accession LN492853 [GenBank]
Sequencing primer gene specific primer, MG10: 5'-AAACAATAATTGGTCTTGGGTCTG-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K031455-022-686-D07C-MG10
CCACACAAAACAAAAATTGTCTTGCCTTTATATGGAATGAAGAAAAATCGAATTTTGTAA
CCATTTTTGACTTTAAAGTCACACTCGCCGTATACGGCACACCAAATGACCAAAAACATT
TGACCACCAACTTTGCTTTAATGTTAAAACTTTTTTTTTGCATCCCACTACTCTCTTCTT
CACCTCCTCAAGTCACAGTGACCAAAATCACACACTTCACAAGAAACAACCTATGGATCC
AAATGAATCTCCACCAAACCAATTTCGCCACGTGGTGGCCATGCCTTATCCAGGTCGAGG
ACACATCAACCCTATGATGAACCTCTGCAAACGCCTTGTCCGTCGATACCCTAACCTTCA
CGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAA
GAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAA
TGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAA
TAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCACTCCCCAAA
TG
   
Sequence Name 07-K031455-022-686-D07C-8409
GenBank Accession LN489150 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K031455-022-686-D07C-8409
GGTATCGACGGACAAGGCGTTTGCAGAGGTTCATCATAGGGTTGATGTGTCCTCGACCTG
GATAAGGCATGGCCACCACGTGGCGAAATTGGTTTGGTGGAGATTCATTTGGATCCATAG
GTTGTTTCTTGTGAAGTGTGTGATTTTGGTCACTGTGACTTGAGGAGGTGAAGAAGAGAG
TAGTGGGATGCAAAAAAAAAGTTTTAACATTAAAGCAAAGTTGGTGGTCAAATGTTTTTG
GTCATTTGGTGTGCCGTATACGGCGAGTGTGACTTTAAAGTCAAAAATGGTTACAAAATT
CGATTTTTCTTCATTCCATATAAAGGCAAGACAATTTTTGTTTTGTGTGGCTAATGGCTA
TGTGGCAACATTGATCCAAGTTTGAACCAGACCCAAGACCAATTATTGTTT

Last Updated on 06.12.2018 16:11