Sequences from confirmation amplicon


Confirmation sequences for line 686E02 and hit At2g18100 are shown.
Line ID 686E02
Locus At2g18100 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:7869728
Primers used for PCR 41kh - 8474, show primer details
   
Sequence Name 83-K113549-0022-686-E02C-41kh
GenBank Accession LN514336 [GenBank]
Sequencing primer gene specific primer, 41kh: 5'-CATTGCAAAAGTCAAGTGGAAATA-3'
Sequence (A. th genome BLAST matches underlined) 
>83-K113549-0022-686-E02C-41kh
AATGTGATGGGCTAGTGGAACGTGGAGGCATAGTTGGAGCGGCTGCTTTAACAGGGGGTA
CATTGATGGCCATCACTGGTGGTATGATGTCTTCTAACTGTTAATATCTCTTCCAACGTT
TAGAAAATTAAGAGTATGTGTTTGGAAAATGCTAATTCTTAATCTGTTTTGATTACATAT
TATGTGAAACAGGATTGGCTGCTCCAGCAATAGCTGCAGGGTTCGGTGCATTGGCTCCAA
CTCTAGGCACAATAGTTCCGGTAATTGGAGCCAGTGGGTTTGCTGCAGCTGCTGAGGCTG
CCGGAACTGTTGCTGGTTCTGTTGCTGTTGCAGCATCTTTTGGAGGTAAATAAAAAGTAG
AATCAGTTTTAGATTCCTTTAAGAAATAAAATGAATCAAAGTGATGATAATTGTATAGTT
CTTAGTCTTCTGTTTTGTTTCTACATGTGTTCATGAATAGCTGCTGGAGCTGGGCTCACT
GGGACGAAGATGGCTAGGAGAACTGGTGACATTGAAGAGTTTGAATTCAAAGCTATTGGA
GAAAATCATAATCAAGGAGTTAGTTTTAAATTCCTGTCCGGGAAATCTACATGGATCAGC
AATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATT
CAAAAATGTAGATGTCCGCA
   
Sequence Name 18-K113548w-0022-686-E02C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K113548w-0022-686-E02C-8409
CTCCTTGATATGATTTTCTCCATAGCTTTGAATTAAACTCTTCAATGTCACCAGTTCTCC
TAGCCATCTTCGTCCCAGTGAGCCCAGCTCCAGCAGCTATTCATGAACACATGTAGAAAC
AAAACAGAAGACTAAGAACTATACAATTATCATCACTTTGATTCATTTTATTTCTTAAAG
GAATCTAAAACTGATTCTACTTTTTATTTACCTCCAAAAGATGCTGCAACAGCAACAGAA
CCAGCAACAGTTCCGGCAGCCTCAGCAGCTGCAGCAAACCCACTGGCTCCAATTACCGGA
ACTATTGTGCCTAGAGTTGGAGCCAATGCACCGAACCCTGCAGCTATTGCTGGAGCAGCC
AATCCTGTTTCACATAATATGTAATCAAAACAGATTAAGAATTAGCATTTTCCAAACACA
TACTCTTAATTTTCTAAACGTTGGAAGAGATATTAACAGTTAGAAGACATCATACCACCA
GTGATGGCCATCAATGTACCCCCTGTTAAAGCAGCCGCTCCAACTATGCCTCCACGTTTC
CACTTAGCCCATTCACTATNTGGTGACAAAGACTCTTCTTCCTTATTTCCACTTGACTGT
TGCAATG

Last Updated on 06.12.2018 16:11