SimpleSearch - Line and FST details


Line specific information

 
Line ID 686E02
Vector Used pAC161
Line Availability available as T3 set from NASC (N465810)
Segregation Analysis 50:42:39
Confirmed for Hit At2g18100
Parent of DUPLO pair 2705
Parent of pair(s) none

Gene hit At5g42325

 
Sequence (A. th genome BLAST matches underlined) 
>13-K023073-022-686-E02-8409
ACATTGAGTATATCCTGATAGTTTACACCGAAGGCGGGAAACGACAATCTGATCCCCGGG
TACGGGATCCTCCCTATTTCGCGCCTCTTTCGTTGCTTTTGAAACCCTAGTTATTACCGA
GAGATCAAACAGAGAACGATGCAGAGGCGAGAGTTCTTGG
GenBank Accession BX662142 [GenBank]
Graphic View Graphic view of gene At5g42325
Predicted Position of Insertion Chr5:16924958 - go to primer design
BLAST e Value 6e-36
Hit Clone Code (BAC ID) K5J14
Hit Gene Code At5g42325 [Araport] [TAIR] [MIPS] [SIGnAL]
Gene Annotation Transcription factor IIS protein
Insertion Classification 5' region
Confirmation Status unknown

Gene hit At2g18100

 
Sequence (A. th genome BLAST matches underlined) 
>13-K022973-022-686-E02-8409
ATGATCCCCGGGTTCCAACAATTACTAATTTCTCTCCTCTTTCGTTGCTTTTGAAACCCT
AGTTATTACCGAGAGATCAAACAGAGAACGATGCAGAGGCGAGAGTTCTTGGAGTTGTTC
GAGGCAGCACTTAGAGCGGCTAAGTCTGTGAAAGGTGCTGAGAGTTCTCCAGAGGTATTG
AGGGTTGCCCATGCTATGCTTCAATTGATAGAGCAGCCTATGTAACTGGATCGAACCCAC
TGGATCCAATTACCGGAGCTATTGTGCCTAGAGTTGGAGCCAATGCACCGAACCCTGCAG
CTATTGCTGGAGCAGCCAATCCTGTTTCACATAATATGCAATCAAAACAGATTAAGAATT
AGCATTTTCCAAACACATACTCTTAATTTTCTAAACGTTGGAAGAGATATTANCAGTTAG
AAGACATCATACCACCAGCGATGGTCATCGACGTATG
GenBank Accession BX662141 [GenBank]
Graphic View Graphic view of gene At2g18100
Predicted Position of Insertion Chr2:7869712 - go to primer design
BLAST e Value 3e-105
Hit Clone Code (BAC ID) T27K22
Hit Gene Code At2g18100 [Araport] [TAIR] [MIPS] [SIGnAL]
Gene Annotation transmembrane/coiled-coil protein (DUF726)
Insertion Classification CDSi
Confirmation Status confirmed, show confirmation sequences
Primer and wt-amplicons show primer details
Other FSTs Supporting this Hit FR814555 [GenBank]


Last Updated on 10.06.2021 13:37