Sequences from confirmation amplicon


Confirmation sequences for line 686F11 and hit At1g14970 are shown.
Line ID 686F11
Locus At1g14970 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:5164719
Primers used for PCR BK68 - 8474, show primer details
   
Sequence Name 40-K104858-0022-686-F11C-BK68
GenBank Accession LN503700 [GenBank]
Sequencing primer gene specific primer, BK68: 5'-TTTGACAAGTTTGCCAGACAATAG-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K104858-0022-686-F11C-BK68
TCATTTGATGTAAGATGATTAACCTACCGAAGTCCGCTGCTGATTCAAGCCTCCATTTGC
CTCAATAAATATGAAACCATTTGACACAGGTAAAACTACAATTGTTAACACAAACAACAA
CAAGAAAGCCTAATTGATTCAACTTATTACTTAGGACAAAGAACACTAACGAAGAAACTA
GGAGAACAATGTAAAATACCTCCAGTAGACATGTTCACACATGGCTTCCATTCTACACCT
TTATAGGAACGCTTCCAAATTGTTGATATCTTAAATGAATACAATTGCATTGACGATACA
TGACAAATGGGAACAAGAAAAGAACAGAGATCAATCTATGTTCAACATTGTTATACGTTC
TCCTAGATATTATACTCTAATTGGATCAATCTATGTTCGACAATGGCCACATTCTCAAAA
CGATATCACAAGCGGGATAAAACGGAGGCAAACCTCACCGCATCAGCCGTGGTATTATCG
GCTTCAATCTCGCCGCGAAGCCTAGCGTAAACCTGAGGGCTCCGGTAGACGGATCCCGGA
GCAGGACGGCGGTGGATAATGGGAGAGGCATCAAACGACGCCGCGTGTAGGTGAAAAAGC
ATACAGGAGATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCAAAAATGTAG
   
Sequence Name 36-K104859-0022-686-F11C-OriL
GenBank Accession LN502216 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K104859-0022-686-F11C-OriL
TTTTCCCTACCGCGGCGTCGTTTGATGCCTCTCCCATTATCCACCGCCGTCCTGCTCCGG
GATCCGTCTACCGGAGCCCTCAGGTTTACGCTAGGCTTCGCGGCGAGATTGAAGCCGATA
ATACCACGGCTGATGCGGTGAGGTTTGCCTCCGTTTTATCCCGCTTGTGATATCGTTTTG
AGAATGTGGCCATTGTCGAACATAGATTGATCCAATTAGAGTATAATATCTAGGAGAACG
TATAACAATGTTGAACATAGATTGATCTCTGTTCTTTTCTTGTTCCCATTTGTCATGTAT
CGTCAATGCAATTGTATTCATTTAAGATATCAACAATTTGGAAGCGTTCCTATAAAGGTG
TAGAATGGAAGCCATGTGTGAACATGTCTACTGGAGGTATTTTACATTGTTCTCCTAGTT
TCTTCGTTAGTGTTCTTTGTCCTAAGTAATAAGTTGAATCAATTAGGCTTTCTTGTTGTT
GTTTGTGTTAACAATTGTAGTTTTACCTGTGTCAAATGGTTTCATATTTATTGAGGCAAA
TGGAGGCTTGAATCAGCAGCGGACTTCGGTAGGTTAATCATCTTACATCAAATTGATTTT
TTTCACACAAGCCTTTCTTGCTATTGTCTG
Primers used for PCR BK69 - 8474, show primer details
   
Sequence Name 39-K104858-0022-686-F11C-BK69
GenBank Accession LN503345 [GenBank]
Sequencing primer gene specific primer, BK69: 5'-GCATTGAATTCTCACGTTTAACAA-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K104858-0022-686-F11C-BK69
GATTGAAGAAGAAAAGACTAAAGAACTATAACACATAAGAAAAGCCAAATAAATACTCAA
GTACATGTGATTAATCGATCATGCTTCCACCGCACGAGCGCATACATACATAACATAAAT
AAAATTTCTCTCTCTTTTGCTTACTTCACTCACAGTTTCGCCGCCGGGATAAATCCGTTC
AATCGGCGTTTGCCACCTGCCATTGTTACAAGCAATGCATTATATCGTTAATGGCGTAGC
GCATTGAGAAGAGAGAGAGAGAGCAGGGAAACAATGATTTCCCAGGTGGAGAGAGATCTG
TCGATTCAAAATCGGCTTCCGGGAAGCGATCACACTACTCCTTCTCCGCCAACATCTCCT
CATCTCTGCCGGAGCCGGTCGAAATCCTCCTCTGTCTCCGGGCAACAGCAAAGTCGGAAC
GTCGCTCACCGTCTCTCATGGATCATTTTATCTGTTCTTCTCCGGCGTCAGGATATATTC
AATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCA
ATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATTC
   
Sequence Name 35-K104859-0022-686-F11C-8409
GenBank Accession LN501825 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K104859-0022-686-F11C-8409
TTTACAATTGAATATATCCTGACGCCGGAGAAGACAGATAAAATGATCCATGAGAGACGG
TGAGCGACGTTCCGACTTTGCTGTTGCCCGGAGACAGAGGAGGATTTCGACCGGCTCCGG
CAGAGATGAGGAGATGTTGGCGGAGAAGGAGTAGTGTGATCGCTTCCCGGAAGCCGATTT
TGAATCGACAGATCTCTCTCCACCTGGGAAATCATTGTTTCCCTGCTCTCTCTCTCTCTT
CTCAATGCGCTACGCCATTAACGATATAATGCATTGCTTGTAACAATGGCAGGTGGCAAA
CGCCGATTGAACGGATTTATCCCGGCGGCGAAACTGTGAGTGAAGTAAGCAAAAGAGAGA
GAAATTTTATTTATGTTATGTATGTATGCGCTCGTGCGGTGGAAGCATGATCGATTAATC
ACATGTACTTGAGTATTTATTTGGCTTTTCTTATGTGTTATAGTTCTTTAGTCTTTTTCT
TTCTTCAATTCTTTGATTTCAGTTTCTATGGAAAACTATTGTTAAACGT

Last Updated on 06.12.2018 16:11