Sequences from confirmation amplicon


Confirmation sequences for line 687A01 and hit At1g03440 are shown.
Line ID 687A01
Locus At1g03440 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:852823
Primers used for PCR MF09 - 8474, show primer details
   
Sequence Name 01-K031753-022-687-A01C-8409
GenBank Accession LN486454 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K031753-022-687-A01C-8409
CATTTACTGAAGCCAGTTCTTCGAGTCTTGGGATTTCACTTCTGATCCATGCGGCTTCGC
TGGCGTCTACTGCAACGGAGATAAAGTAATCTCTCTCAATCTCGGCGATCCTAGAGCCGG
TTCACCCGGTTTATCGGGTCGGATCGACCCAGCAATAGGCAAACTCTCTGCACTCACTGA
GCTCTCCATTGTCCCCGGCAGAATCATGGGTGCGTTACCGGCAACAATCTCTCAGCTAAA
AGACCTTCGTTTTCTCGCAATCAGCCGGAATTTCATCTCCGGCGAGATTCCGGCGAGTCT
CGGCGAGGTTCGTGGTCTCAGAACACTGGATTTAAGCTATAATCAATTAACCGGAACTAT
CTCTCCGTCAATCGGATCGTTACCGGAGCTCTCCAATTTGATTCTCTGCCACAACCACTT
AACCGGATCAATACCACCGTTTCTCTCACAAACTCTAACCCGAAT
   
Sequence Name 02-K031753-022-687-A01C-MF09
GenBank Accession LN486915 [GenBank]
Sequencing primer gene specific primer, MF09: 5'-ATTCGGGTTAGAGTTTGTGAGAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K031753-022-687-A01C-MF09
AATTGGAGAGCTCCGGTAACGATCCGATTGACGGAGAGATAGTTCCGGTTAATTGATTAT
AGCTTAAATCCAGTGTTCTGAGACCACGAACCTCGCCGAGACTCGCCGGAATCTCGCCGG
AGATGAAATTCCGGCTGATTGCGAGAAAACGAAGGTCTTTTAGCTGAGAGATTGTTGCCG
GTAACGCACCCATGATTCTGCCGGGGACAATGGAGAGCTCAGTGAGTGCAGAGAGTTTGC
CTATTGCTGGGTCGATCCGACCCGATAAACCGGGTGAACCGGCTCTAGGATCGCCGAGAT
TGAGAGAGATTACTTTATCTCCGTTGCAGTAGACGCCAGCGAAGCCGCATGGATCAGAAG
TGAAATCCCAAGACTCGAAGAACTTGGCTTCATGTAAATGGCTTCATGTCCGGGAAATCT
ACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAAT
TTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTT
GATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTC
TAATTCGGAAATCTTTATTTCGACGTGTCTACATTCACGTCCCAATG
Primers used for PCR MF10 - 8474, show primer details
   
Sequence Name 25-K031754-022-687-A01C-8409
GenBank Accession LN497323 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K031754-022-687-A01C-8409
CTTTACAATTGAATATATCCTGGCAAATCATCGAGGGATTTACGAATAGCTTGCAGAGCC
AAGAAATCAACTGGGTCGAGAATCGCCTCCGTGAAGAAGCCGAATCGAAGCAGGAACATC
AAACACCAAACCAACTTGGTAAATCTCATCATCATCTTCTTCTTCTTCTTCTTCAGACTT
AGCCGTTACAGGTTCAAGAAACAGAGACTATATATATACACAGAGGGAAGAACAGAGACG
AGCTGTAAAGTAGAGAAAATGGGTTTTGTTTTCTGATTTCTCGTGAGAGGGAAAGTCTCT
GGTTTATGTGGTGGAAAGAAGGTGT
   
Sequence Name 26-K031754-022-687-A01C-MF10
GenBank Accession LN497801 [GenBank]
Sequencing primer gene specific primer, MF10: 5'-ACACCTTCTTTCCACCACATAAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K031754-022-687-A01C-MF10
CAAAACCCATTTTCTCTACTTTACAGCTCGTCTCTGTTCTTCCCTCTGTGTATATATATA
GTCTCTGTTTCTTGAACCTGTAACGGCTAAGTCTGAAGAAGAAGAAGAAGAAGATGATGA
TGAGATTTACCAAGTTGGTTTGGTGTTTGATGTTCCTGCTTCGATTCGGCTTCTTCACGG
AGGCGATTCTCGACCCAGTTGATTTCTTGGCTCTGCAAGCTATTCGTAAATCCCTCGATG
ATTTGCCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGAC
AAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAA
TCTTTATTTCGACGTGTCTACATTCACTCCCAAATGGG

Last Updated on 06.12.2018 16:11