Genotyping and primer details


Genotyping and primer details for 687A01 - At1g03440
Line ID 687A01
Locus At1g03440 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:852823 of line 687A01 Chr1:852823
   
Insertion allele amplicon 8474 - MF09
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer MF09: 5'-ATTCGGGTTAGAGTTTGTGAGAGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:853273
   
Insertion allele amplicon 8474 - MF10
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer MF10: 5'-ACACCTTCTTTCCACCACATAAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:852511
   
wt amplicon (over InsPos) MF10 - MF09
wt amplicon start Chr1:852511
wt amplicon end Chr1:853273
wt amplicon size 763 bp
Locus specific primer MF09: 5'-ATTCGGGTTAGAGTTTGTGAGAGA-3'
2nd locus specific primer MF10: 5'-ACACCTTCTTTCCACCACATAAAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11