Sequences from confirmation amplicon


Confirmation sequences for line 687C03 and hit At2g37130 are shown.
Line ID 687C03
Locus At2g37130 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:15598239
Primers used for PCR AP29 - 8474, show primer details
   
Sequence Name 73-K106402-0022-687-C03C-8409
GenBank Accession LN512905 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K106402-0022-687-C03C-8409
TTACAATTGAATATTGACAATCCTTCCTGATCTCCCCTTGGTCTCCCGTGAGCGGGTTGG
TCTCGGACAAGAGCCTGACGCCACGTGAGAACTGCTCATGGAAGTAATTATTGTCCGCAG
CCATCTTGGCCACAAACGGTGCGGTCCTGGGATCGGTGGCTAGCTCATCATCAATGACAA
GAAGCCCCTTATGGGCCATGATGTTCTTATAATACATGTTGTCCACAACCATCGGAGTCT
CACGATCGTTACGGGAGTACAAGACGGCGTTCGGGTCCGGTGTTGGACTTGGGCAACGTT
TTTTTAAGTAAAGGGCGTAACTTGGGTCGAGAGTAGGGTCAATCGTTGGGTATAGCCGGT
GCACTAGGTTAACGCAGTGGACCCTACCCACTGAGTGAGCACCTACGGTTATATACATTG
ACTGATCACTAACAATATCATTTTATATTAAAAAAAGAAAAAAAAACAATATCATTTTAT
AATTAAAAATAAAATAAAATAACGATATCATCCTTATTTAAGGAAATTCAATTAGTATCT
CTAAGAAAATTTCTGTGATATATCTTTTTACTTCACTAGACATTACATCTCCAAA
   
Sequence Name 89-K106403-0022-687-C03C-AP29
GenBank Accession LN514956 [GenBank]
Sequencing primer gene specific primer, AP29: 5'-GTTTGGTATTTGATTCGGAGATGT-3'
Sequence (A. th genome BLAST matches underlined) 
>89-K106403-0022-687-C03C-AP29
TTTCTAGAGATCTAATTGAATTTCCTTAAATAAGGATGATATCGTTATTTTATTTTATTT
TTAATTATAAAATGATATTGTTTTTTTTTCTTTTTTAATATAAAATGATATTGTTAGTGA
TCAGTCAATGTATATAACCGTAGGTGCTCACTCAGTGGGTAGGGTCCACTGCGTTAACCT
AGTGCACCGGCTATACCCAACGATTGACCCTACTCTCGACCCAAGTTACGCCCTTTACTT
AAAAAAACGTTGCCCAAGTCCAACACCGGACCCGAACGCCGTCTTGTACTCCCGTAACGA
TCGTGAGACTCCAATGGTTGTGGACAACATGTATTATAAGAACATCATGGCCCATAAGGG
GCTTCTTGTCATTGATGATGAGCTAGCCACCGATCCCAGGACCGCACCGTTTGTGGCCAA
GATGGCTGCGGACAATAATTACTTCCATGAGCAGTTCTCACGTGGCGTCAGGCTCTTGTC
CGAGACCAACCCGCTCACGGGAGACCAAGGGGAGATCAGGAAGGATTGTCAATATTCAAT
TGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG

Last Updated on 06.12.2018 16:11