Sequences from confirmation amplicon


Confirmation sequences for line 687C04 and hit At5g12850 are shown.
Line ID 687C04
Locus At5g12850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:4059079
Primers used for PCR 94pq - 8474, show primer details
   
Sequence Name 22-K117583-0022-687-C04C-94pq
GenBank Accession LN496290 [GenBank]
Sequencing primer gene specific primer, 94pq: 5'-TAAACGAATCATCAGTTTCACAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K117583-0022-687-C04C-94pq
AGCTCAGCGATCTAGGTGCAGCTGTTCAAGCCACGCATCAAGAATGTCGCTCTCACGAGT
GTCACTGTTGTGAGGATTCACACTCGAACCACCTTGAGTCAATGGCCTTGCACCATTCAT
ATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATG
GTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGAT
GTCCGCAGCGTTATTAT
   
Sequence Name 06-K117582-0022-687-C04C-8409
GenBank Accession LN488991 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K117582-0022-687-C04C-8409
GTGCAAGGCCATTGACTCAAGGTGGTTCGAGTGTGAATCCTCACAACAGTGACACTCGTG
AGAGCGACATTCTTGATGCGTGGCTTGAACAGCTGCACCTAGATCGCTGAGCCTCAGCTG
CGAGAGAGAGGTTCACATTTCTGTGAAGCTGTGAAACTGATGNATTCGTTTA

Last Updated on 06.12.2018 16:11