Sequences from confirmation amplicon


Confirmation sequences for line 692F02 and hit At2g40390 are shown.
Line ID 692F02
Locus At2g40390 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:16868199
Primers used for PCR 38vf - 8474, show primer details
   
Sequence Name 29-K117753-0022-692-F02C-38vf
GenBank Accession LN499463 [GenBank]
Sequencing primer gene specific primer, 38vf: 5'-AACCTAGGCTCCACTTCTAACCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K117753-0022-692-F02C-38vf
TCTGCATCACGAGGCGCCAACGAGCCTGAGAACAACTTGTCTCAAAACGGTGAAAGATCA
GCTGGTAACTTGTAGGTCAAGGCAGGGCTCTAAGTTGCTCATGGTGCAACTAGGCTCTAA
CCTTGAAGAACAATGGATGAGATCGCTGAACCTTGCAATAACCAACTGGATCATCGAGAT
CAAAGCGTTTCAGCATCTCAAGTCTCCTTCTCCTTTGTTTTCATATGCTTTCTCGACTCA
AGGTCTATGGAAAGTTCATATGTATTGTCCTGTGGTAGCAATGGAAATGGAGAGCGTAAA
TAGTTCTCTAAACGACGAGAGATTGTTCTTCTCGCTAAACTATCATCAACTTGAAGGTGT
AATCCAATTGAACCACAGGATTTACGTTCGTGAAAAATATTACCAATGAGTAAATATCTA
CATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATT
TTTTTTCAATTCAAAAATGTAGATGTC
   
Sequence Name 13-K117752-0022-692-F02C-8409
GenBank Accession LN492234 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K117752-0022-692-F02C-8409
TTTTTACGAACGTAAATCCTGTGGTTCAATTGGATTACACCTTCAAGTTGATGATAGTTT
AGCGAGAAGAACAATCTCTCGTCGTTTAGAGAACTATTTACGCTCTCCATTTCCATTGCT
ACCACAGGACAATACATATGAACTTTCCATAGACCTTGAGTCGAGAAAGCATATGAAAAC
AAAGGAGAAGGAGACTTGAGATGCTGAAACGCTTTGATCTCGATGATCCAGTTGGTTATT
GCAAGGTTCAGCGATCTCATCCATTGTTCTTCAAGGTTAGAGCCTAGTTGCACCATGAGC
AACTTAGAGCCCTGCCTTGACCTACAAGTTACCAGCTGATCTTTCACCGTTTTGAGACAA
GTTGTTCTCAGGCTCGTTGGCGCCTCGTAGATGCAGATGAGGAACACGAAAGTGAAGAAA
GCGAGGTTGAAAACGTCTTTGAGGTTAGA

Last Updated on 06.12.2018 16:11