Sequences from confirmation amplicon


Confirmation sequences for line 692H03 and hit At2g45720 are shown.
Line ID 692H03
Locus At2g45720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:18834766
Primers used for PCR 32sf - 8474, show primer details
   
Sequence Name 41-K116518-0022-692-H03C-32sf
GenBank Accession LN504220 [GenBank]
Sequencing primer gene specific primer, 32sf: 5'-CATCAAGGCATATTTGATCCTTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K116518-0022-692-H03C-32sf
TCGCTAAGTTTTTGTTTTTGTTTTCTTCTTTTGTGCAATTCAGTGCTACGGAGTGCAAGG
ATTGTATTTGTGTGTGATCATGGTGGAGGAGAAAACGGGAAACGTGACTCTACTTGATCA
AACAGTTGAAGATTTGTTATTACAGGCACAAGAGCTTGTACCGATCGCTTTGTCCAAAGC
AAGAACAGTGAAAGGTTTCTCAAGCAGATGGAGAGTGATCATTTCAAGATTAGAGAAGAT
ACCGACATGTTTATCGGATTTGTCTAGCCATCCTTGCTTCTCTAAGCACACTCTATGCAA
AGAGCAACTTCAAGCAGTCTTAGAAACTTTGAAAGAAACCATCGAGCTCGCGAATGTTTG
CGTAAGTGAGAAGCAAGAAAAATAAAATGATCAAATCTACATGGATTATATCCAATTGTA
AATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGA
GAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTACGCA
   
Sequence Name 15-K116517-0022-692-H03C-8409
GenBank Accession LN493139 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K116517-0022-692-H03C-8409
TTGGATATAATCCATGTAGATTTGATCATTTTATTTTTCTTGCTTCTCACTTACGCAAAC
ATTCGCGAGCTCGATGGTTTCTTTCAAAGTTTCTAAGACTGCTTGAAGTTGCTCTTTGCA
TAGAGTGTGCTTAGAGAAGCAAGGATGGCTAGACAAATCCGATAAACATGTCGGTATCTT
CTCTAATCTTGAAATGATCACTCTCCATCTGCTTGAGAAACCTTTCACTGTTCTTGCTTT
GGACAAAGCGATCGGTACAAGCTCTTGTGCCTGTAATAACAAATCTTCAACTGTTTGATC
AAGTAGAGTCACGTTTCCCGTTTTCTCCTCCACCATGATCACACACAAATACAATCCTTG
CACTCCGTAGCACTGAATTGCACAAAAGAAGAAAACAAAAACAAAAACTTTAGCGATTTG
TAGAAACAAAAAACCAAAGCCAAAAAGGATCAAAAGGCCCTTGATG

Last Updated on 06.12.2018 16:11