Sequences from confirmation amplicon


Confirmation sequences for line 693A12 and hit At5g43720 are shown.
Line ID 693A12
Locus At5g43720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:17558840
Primers used for PCR 13ih - 8474, show primer details
   
Sequence Name 40-K112810-0022-693-A12C-13ih
GenBank Accession LN503761 [GenBank]
Sequencing primer gene specific primer, 13ih: 5'-CTCTCAACTCTTCCTCCTTCTTCA-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K112810-0022-693-A12C-13ih
CGCAGGAGGCTATGCCAAGAGAAGGGATTCGGAACCAAAACAGACGGCGGGAAGCAGTCG
GAGGTCGAAAGTATTGCGTGTAGAGAAGAAACCTAAGACTGTCTCCATCAAGAATCAGAT
GCGTTCTGTTGAACGCTTTCTTCGTAAAGTCAGTTCCTTTTCTCTGTAAATTAGTGTTTT
TTGGTAGAATTTGTGTTTAAGTTCCGGTCTTTCTGGTGACTCAGTTAGCTTGTCTAGTGT
AGAGCTTAAGGTTGATAATGTCATTTCTAGGTTGTGTTTTTGTAGAGAATTTGATAATCT
ATGGTCTATTAGAAGCAAACCCCATATGAGAATATTGAATGTTGATGTATTTGATACTTT
TATAGGATTTGCCTCCTGAAGTAAGAGAGTCTCTAGTAAATGGCTTCATGTCCGGGAAAT
CTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCA
ATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 15-K112809-0022-693-A12C-8409
GenBank Accession LN493046 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K112809-0022-693-A12C-8409
GCATTTACAGAGACTCTCTTACTTCAGGAGGCAAATCCTATAAAAGTATCAAATACATCA
ACATTCAATATTCTCATATGGGGTTTGCTTCTAATAGACCATAGATTATCAAATTCTCTA
CAAAAACACAACCTAGAAATGACATTATCAACCTTAAGCTCTACACTAGACAAGCTAACT
GAGTCACCAGAAAGACCGGAACTTAAACACAAATTCTACCAAAAAACACTAATTTACAGA
GAAAAGGAACTGACTTTACGAAGAAAGCGTTCAACAGAACGCATCTGATTCTTGATGGAG
ACAGTCTTAGGTTTCTTCTCTACACGCAATACTTTCGACCTCCGACTGCTTCCCGCCGTC
TGTTTTGGTTCCGAATCCCTTCTCTTGGCATAGCCTCCGTGCGCCATGGCTGAAGGAGTT
TGCTAAAGCTTTTTAGAAGTTGAAGAAGGAGGAAAGGTTGAGAG

Last Updated on 06.12.2018 16:11