Sequences from confirmation amplicon


Confirmation sequences for line 694B11 and hit At1g70090 are shown.
Line ID 694B11
Locus At1g70090 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:26401925
Primers used for PCR 30jn - 8474, show primer details
   
Sequence Name 57-K113934-0022-694-B11C-30jn
GenBank Accession LN509484 [GenBank]
Sequencing primer gene specific primer, 30jn: 5'-TATTCTTGATCCATGCGTAGACAG-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K113934-0022-694-B11C-30jn
TAACAAAGCTTTGGAACCGAGTTTGACAGGGTCAAGAATCATCGGAGCTCCGGAGTATTG
TCACGCTAACTTCACAAAGTACTTCACTTCAGGTTTCTGGTCCGACCCGGCTTTACCCGG
TTTCTTCTCGGGTCGAAAGCCTTGTTATTTCAACACGGGTGTGATGGTGATGGATCTAGT
TAGATGGAGAGAAGGAAACTACAGAGAAAAGCTTGAAACTTGGATGCAGATACAGAAGAA
GAAGAGAATCTACGATTTGGGTTCTTTGCCTCCGTTTCTTCTTGTCTTCGCAGGGAACGT
TGAAGCAATTGATCATAGGTGGAACCAACATGGTTTAGGAGGAGACAATGTACGAGGAAG
TTGTAGGTCTTTGCATAAAGGACCAGTGAGTTTGTTGCATTGGAAGAGACCAGCAATGGC
TTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAA
GAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACAGTTTATT
   
Sequence Name 22-K113933-0022-694-B11C-8409
GenBank Accession LN496201 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K113933-0022-694-B11C-8409
GGTCTCTTCCATGCACAAACTCACTGGTCCTTTATGCAAAGACCTACAACTTCCTCGTAC
ATTGTCTCCTCCTAAACCATGTTGGTTCCACCTATGATCAATTGCTTCAACGTTCCCTGC
GAAGACAAGAAGAAACGGAGGCAAAGAACCCAAATCGTAGATTCTCTTCTTCTTCTGTAT
CTGCATCCAAGTTTCAAGCTTTTCTCTGTAGTTTCCTTCTCTCCATCTAACTAGATCCAT
CACCATCACACCCGTGTTGAAATAACAAGGCTTTCGACCCGAGAAGAAACCGGGTAAAGC
CGGGTCGGACCAGAAACCTGAAGTGAAGTACTTTGTGAAGTTAGCGTGACAATACTCCGG
AGCTCCGATGATTCTTGACCCTGTCAAACTCGTGTTCCAAAGCTTTGTTATGTCATCGAC
GACGATGATGTCCGAGTCTAGGTAAATGACTCTGTCTACGCATGGACCAAGAAT

Last Updated on 06.12.2018 16:11