Sequences from confirmation amplicon


Confirmation sequences for line 694C03 and hit At2g45960 are shown.
Line ID 694C03
Locus At2g45960 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:18910027
Primers used for PCR 23kk - 8474, show primer details
   
Sequence Name 43-K113872-0022-694-C03C-23kk
GenBank Accession LN504910 [GenBank]
Sequencing primer gene specific primer, 23kk: 5'-CGTAGCTATAAACTCAGCAATCCC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K113872-0022-694-C03C-23kk
CCGGGCTCGACAACGGCGCAGGTGGTGGCTCTTTGTAGTCCTTGTCACTCTGAGCCGAAG
TTCCGATCGGTTGCCTCTCCGGAAACTTGTTAGCTCCGACTCTAACATCTTCTTCTTTAC
CTTCCATCTCTCTCTCTCTTTCTCTCTAGAGCTTTCTAGGTTTTTCTGTGAGTGGGTTAT
GTGTTGCAGAGAAGCAGAGAGAGTGGTGGCTTTTTATAGTGACCGAAACTCAAGGTTGGA
AGATGATGGAAACAAAATTAAAACATAAACAAGATGATAGTTAATTAGTTAACTTAATTG
CATGATAGTATAATTAACGTTATCATCTTTGCTGCTGCTGAATACTGATAATGCTTGTTA
TTGATTAATGATAATTAAAGTATGTGGACAGAGTTTCAAATCCCGCTTTGCAACTATTTT
CTTTACTCTAATCAAATGTCGTTATTGTACCCTAATAAAATAAATAAAATTGTGGACATT
TTTTAACGTTTAAATGTAGATTATGACAGGTAAGAAAATACAGAAAATCATATTAAAAAA
GCAGTTGAATCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAG
TATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAA
TGTAGATGTCCC
   
Sequence Name 17-K113871-0022-694-C03C-8409
GenBank Accession LN493932 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K113871-0022-694-C03C-8409
ATTGATTCACTGCTTTTTAATATGATTTTCTGTATTTTCTTACCTGTCATAATCTACATT
TAAACGTTAAAAAATGTCCACAATTTTATTTATTTTATTAGGGTACAATAACGACATTTG
ATTAGAGTAAAGAAAATAGTTGCAAAGCGGGATTTGAAACTCTGTCCACATACTTTAATT
ATCATTAATCAATAACAAGCATTATCAGTATTCAGCAGCAGCAAAGATGATAACGTTAAT
TATACTATCATGCAATTAAGTTAACTAATTAACTATCATCTTGTTTATGTTTTAATTTTG
TTTCCATCATCTTCCAACCTTGAGTTTCGGTCACTATAAAAAGCCACCACTCTCTCTGCT
TCTCTGCAACACATAACCCACTCACAGAAAAACCTAGAAAGCTCTAGAGAGAAAGAGAGA
GAGAGATGGAAGGTAAAGAAGAAGATGTTAGAGTCGGAGCTAACAAGTTTCCGGAGAGGC
AACCGATCGGAACTTCGGCTCAGAGTGACAAGGACTACAAAGAGCCACCACCTGCGCCGT
TGTTCGAGCCCGGCGAGCTAGCTTCATGGTCCTTCTGGAGAGCTGGGATTGCTGAGTTAT
AAGCTACG

Last Updated on 06.12.2018 16:11