Sequences from confirmation amplicon


Confirmation sequences for line 694C03 and hit At3g25860 are shown.
Line ID 694C03
Locus At3g25860 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:9460878
Primers used for PCR 30lu - 8474, show primer details
   
Sequence Name 32-K113947-0022-694-C03C-30lu
GenBank Accession LN500668 [GenBank]
Sequencing primer gene specific primer, 30lu: 5'-CCGTCCACTTCACTCTCTCTAAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K113947-0022-694-C03C-30lu
CACATGGCGGTTTCTTCTTCTTCGTTTCTATCGACAGCTTCACTAACCAATTCCAAATCC
AACATTTCATTCGCTTCCTCAGTATCCCCATCCCTCCGCAGCGTCGTTTTCCGCTCCACG
ACTCCGGCGACTTCTCACCGTCGTTCAATGACGGTCCGATCTAAGATTCGTGAAATTTTC
ATGCCGGCGTTATCATCAACCATGACGGAAGGCAAAATCGTGTCATGGATCAAAACAGAA
GGCGAGAAACTGAATAAACGACAATATGGCTTCATGTCCGGGAAATCTACATGGATCAGC
AATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATT
CAAAAATGTAGATGTCCG
   
Sequence Name 04-K113946-0022-694-C03C-8409
GenBank Accession LN488000 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K113946-0022-694-C03C-8409
TCGTTTATTCGTTTCTCGCCTTCTGTTTTGATCCATGACACGATTTTGCCTTCCGTCATG
GTTGATGATAACGCCGGCATGAAAATTTCACGAATCTTAGATCGGACCGTCATTGAACGA
CGGTGAGAAGTCGCCGGAGTCGTGGAGCGGAAAACGACGCTGCGGAGGGATGGGGATACT
GAGGAAGCGAATGAAATGTTGGATTTGGAATTGGTTAGTGAAGCTGTCGATAGAAACGAA
GAAGAAGAAACCGCCATTGTTGAATCACAGAGAGAGAGAGATCTGAGAGAGAGTTTAGAG
AGAGTGGAGTGGACGG

Last Updated on 06.12.2018 16:11