Sequences from confirmation amplicon


Confirmation sequences for line 697F05 and hit At4g28000 are shown.
Line ID 697F05
Locus At4g28000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:13928738
Primers used for PCR 04fx - 8474, show primer details
   
Sequence Name 60-K111688-0022-697-F05C-04fx
GenBank Accession LN510298 [GenBank]
Sequencing primer gene specific primer, 04fx: 5'-ACCTGTAAACGCGAAACAGTTAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K111688-0022-697-F05C-04fx
CCGCACTATCTTTTTTTACTTTACCTGGCTTTTAGCTACTTTCATGTCTTCCATGCTCAA
AGGTCTCAACGTTATGCCTCTCTCTTCAGATACTTCTTCTTTTGCTTCAGATCCTTCTTC
ACTATTCTTCTCTGCTTCCTCTCTCTTCCTCCTCTCCTGGATTTTATGAGAAACCACCAT
TAAAATACTCATTCTAGCGCTAGAAAGTAGAGAAATAAAGGAAGGACGAGTTTTAGGAGT
TCTTACCTGGTCTTTCAAGCACTCTTGCTTTATCAGCTCCCTCACTGGTCGATATGCTGC
TGTTGTGCAAAAGTTCTGTTTGTATAACATTCACAATAATTAGTTCATAAAGAGAAGATT
CAGATGTTTGTAAAGCGAGATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAG
TATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAA
TGTAGATGTCCGCA
   
Sequence Name 44-K111684-0022-697-F05C-8409
GenBank Accession LN505225 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K111684-0022-697-F05C-8409
CTCGCTTTCAACATCTGAATCTTCTCTTTATGAACTAATTATTGTGAATGTTATACAAAC
AGAACTTTTGCACAACAGCAGCATATCGACCAGTGAGGGAGCTGATAAAGCAAGAGTGCT
TGAAAGACCAGGTAAGAACTCCTAAAACTCGTCCTTCCTTTATTTCTCTACTTTCTAGCG
CTAGAATGAGTATTTTAATGGTGGTTTCTCATAAAATCCAGGAGAGGAGGAAGAGAGAGG
AAGCAGAGAAGAATAGTGAAGAAGGATCTGAAGCAAAAGAAGAAGTATCTGAAGAGAGAG
GCATAACGTTGAGACCTTTGAGCATGGAAGACATGAAAGTAGCTAAAAGCCAGGTAAAGT
TAAAAAAAGATTAGTTGCGGTGTTGATGATGATCATGCGCAAAACACTAACTGTTTCGCG
NTTACAGG

Last Updated on 06.12.2018 16:11