Sequences from confirmation amplicon


Confirmation sequences for line 700B09 and hit At3g56200 are shown.
Line ID 700B09
Locus At3g56200 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:20851024
Primers used for PCR 10so - 8474, show primer details
   
Sequence Name 55-K116569-0022-700-B09C-10so
GenBank Accession LN508917 [GenBank]
Sequencing primer gene specific primer, 10so: 5'-GTGGTCCTGAACATATTGGAGTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K116569-0022-700-B09C-10so
GAGATCTTCGCTTTGTTATTTGTCTACGGCTTTGTCTTGCTTCCATTGGTCCTGCGTAGA
CGAGTCGGTAAGTTTCTCTCTATAATCTTGTGTTCTCAAAATTATGGATATCTATGAGAT
TGTTGATGATTTTCTTTTTGTTAATGGTTATGTAGAAAGACTGGCAATTAGTTCCGCTGT
ATCGTTTCTTCTTGCGGTTCTATTTGTTGTCATAAGCTCGGTGCTGGCGATTTCCGCGCT
GGTGAATGGACAAACGAAGAATCCAAGACTGTTTCCGGAGTTGAGCAATGGAGGATCGTT
CTGGCAACTCTTCACAGCTTCCCCTGTTATAGTTACAGCCTTCACGTTTCACTTCAATGG
TAAGAACCCATCTCTTTCACCATAGCTTTCCCCTGTTATATCTCTTCAGGATATATTCAA
TTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAAT
ATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
A
   
Sequence Name 26-K116568-0022-700-B09C-8409
GenBank Accession LN498089 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K116568-0022-700-B09C-8409
CATTTCATTGAATATATCCTGAAGAGATATAACAGGGGAAAGCTATGGTGAAAGAGATGG
GTTCTTACCATTGAAGTGAAACGTGAAGGCTGTAACTATAACAGGGGAAGCTGTGAAGAG
TTGCCAGAACGATCCTCCATTGCTCAACTCCGGAAACAGTCTTGGATTCTTCGTTTGTCC
ATTCACCAGCGCGGAAATCGCCAGCACCGAGCTTATGACAACAAATAGAACCGCAAGAAG
AAACGATACAGCGGAACTAATTGCCAGTCTTTCTACATAACCATTAACAAAAAGAAAATC
ATCAACAATCTCATAGATATCCATAATTTTGAGAACACAAGATTATAGAGAGAAACTTAC
CGACTCGTCTACGCAGGACCAATGGAAGCAAGACAAAGCCGTAGACAAATAACAAAGCGA
AGATTCTCGTGTTCCACCAGTAAGAACCAAACCATTCTTGCAAAACTCCAATATTCAGAG
GACCAC

Last Updated on 06.12.2018 16:11