Sequences from confirmation amplicon


Confirmation sequences for line 701A10 and hit At4g09930 are shown.
Line ID 701A10
Locus At4g09930 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:6228681
Primers used for PCR 25qt - 8474, show primer details
   
Sequence Name 50-K115779-0022-701-A10C-25qt
GenBank Accession LN507278 [GenBank]
Sequencing primer gene specific primer, 25qt: 5'-GATATGAACAAGAAGCAGATGCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K115779-0022-701-A10C-25qt
CATACATTTATGATCTGTCCGTTTGGTCGCTAGTACTCTTTAGCTCACATTCTTTGGTAA
CAGCTGCTGCTTGTCCTTTTGACTTGAATGCCTCGCTTCTGAGGATGCTATTCCCTGTCG
CACTTTTCCCGTTGCCTGAGCAGCCAATAAGGACTAGGGTTCTTGAAGGCTTCATCGGTA
AATCAGACTCACAAGCATCTTCAAAAAACAGAAAACAGAAACTCTGTCAAAGCCCAAATC
TATAAAAGTCATATTAAGAAAAAGAAATGGCTTCATGTCCGGGAAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAGATGTCCG
   
Sequence Name 02-K115778-0022-701-A10C-8409
GenBank Accession LN487172 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K115778-0022-701-A10C-8409
CTTTCTTTTTCTTATATGACTTTTATAGATTTGGGCTTTGACAGAGTTTCTGTTTTCTGT
TTTTTGAAGATGCTTGTGAGTCTGATTTACCGATGAAGCCTTCAAGAACCCTAGTCCTTA
TTGGCTGCTCAGGCAACGGGAAAAGTGCGACAGGGAATAGCATCCTCAGAAGCGAGGCAT
TCAAGTCAAAAGGACAAGCAGCAGCTGTTACCAAAGAATGTGAGCTAAAGAGTACTAAGC
GACCAAACGGACAGATCATAAATGTTATTGACACTCCTGGTGCGTCCATGTGTGTGTTTG
CATCTGCTTCTTGTTCATAT

Last Updated on 06.12.2018 16:11