Sequences from confirmation amplicon


Confirmation sequences for line 701E04 and hit At4g28410 are shown.
Line ID 701E04
Locus At4g28410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:14053377
Primers used for PCR 29st - 8474, show primer details
   
Sequence Name 28-K116478-0022-701-E04C-29st
GenBank Accession LN499002 [GenBank]
Sequencing primer gene specific primer, 29st: 5'-TTCTTCGTCTTCTCCAAGATGTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K116478-0022-701-E04C-29st
CATTTACAGGCACACATTAGCTATTGCGCATTACACGTTGCATTTGTGCATGTGTCATAT
GGAACATTAAGTTTCATAAGAGACAAACCTGTAGGATTGTTGATGGATCTGGACTTATGT
CGAGATGTTCCTTGATGGATTCAACGACCTATTGACAACAAGGTATTGCTCGATGTTCAA
ACAGACTCACGTCTACAAAATGTTAAATTAATATCATGTCCAGTAGGACATTGTTTACTT
CTAATAACGTGTGATGATTGAGAAATTGAAAAACTGACCCTAGTTGTTTTGAAAACGTTT
TTGGGATCGTTCATTGCGATCCAACCTATTCGCCAGCCGGGAACGAGCCATCCCTTGGAT
ATGGACCCGAGTGTAACCACAGGAGTAATGGATGAAAATATCCCCATCGGGACAAATTTA
TTCTCTCCATAAATAGTTTGGTTATAAACTTCATCAGAAATCACCATTATTCCTAGCTTT
CTCGCCACCTCAGCCACCTATACGTCATAATAAAATGTTTAACAAAAGTTTTGTTCAAAG
AAAGTAAATAGTTTTCACTTTTCACGTACTTTCTTTAGATGTTCAATTGTAAATGGCTTC
ATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAA
AGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 03-K116477-0022-701-E04C-8409
GenBank Accession LN487642 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K116477-0022-701-E04C-8409
GCCATTTACATTGAACATCTAAAGAAAGTACGTGAAAAGTGAAAACTATTTACTTTCTTT
GAACAAAACTTTTGTTAAACATTTTATTATGACGTATAGGTGGCTGAGGTGGCGAGAAAG
CTAGGAATAATGGTGATTTCTGATGAAGTTTATAACCAAACTATTTATGGAGAGAATAAA
TTTGTCCCGATGGGGATATTTTCATCCATTACTCCTGTGGTTACACTCGGGTCCATATCC
AAGGGATGGCTCGTTCCCGGCTGGCGAATAGGTTGGATCGCAATGAACGATCCCAAAAAC
GTTTTCAAAACAACTAGGGTCAGTTTTTCAATTTCTCAATCATCACACGTTATTAGAAGT
AAACAATGTCCTACTGGACATGATATTAATTTAACATTTTGTAGACGTGAGTCTGTTTGA
ACATCGAGCAATACCTTGTTGTCAATAGGTCGTTGAATCCATCAAGGAACATCTCGACAT
AAGTCCAGATCCATCAACAATCCTACAGGTTTGTCTCTTATGAAACTTAATGTTCCATAT
GACACATGCACAAATGCAACGTGTAATGCGCAATAGCTAATGTGTTGCCTTGTTTAAATT
GGAAGTTTGCACTTCCAAACATCTTGGAGAGAAACAA

Last Updated on 06.12.2018 16:11