Sequences from confirmation amplicon


Confirmation sequences for line 701G02 and hit At3g24120 are shown.
Line ID 701G02
Locus At3g24120 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:8707570
Primers used for PCR 39vf - 8474, show primer details
   
Sequence Name 62-K117751-0022-701-G02C-39vf
GenBank Accession LN510844 [GenBank]
Sequencing primer gene specific primer, 39vf: 5'-CAAAATCAAGTCTTTGTTTGACGA-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K117751-0022-701-G02C-39vf
TTGTGCCTCTCACATGATATAGTTCTACGAAACTTCTAACAAATATGATTCCAAATAGCC
ATATGAATAAAGAGTAGAACCAAACGGCTTCAAAAACAGAGATATAGACAACAACAACAA
CAAAAAGGTCAACTGAATAGATACCATCTTTAGAGTTCTCAGTTGATTCTTTGCCAGCTT
GCCTCCCTAGGCGGAATTTCTGTCGAGCAAAACACATACAAAAGAGAGAAACACAACACC
ATCAAACACCCAATATTACTATGAATAACACATATAAATGAAACTTAAATCCAACCTAGA
TACAACTCTTAATACCAAAGCAAATTTCAGAATCTAAGGCGTCAAGTCATTGAAATTCTA
GGTGTGGTCTCATCAACCAAGATAGATCTAATAACAGTTAATTAGGAATCAGTTTTGTTT
ATCAAACCTGAAGATGTGATTTGAGGTGGTAGAGAGTGAGACCCTTCACTCCCATTGTTC
TCATAATAGTTTTGGGAGTCGCTTCTGTAAATTGAACAGACAGAGTCAGAACGGTCTCTT
CAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTC
AATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
   
Sequence Name 37-K117750-0022-701-G02C-8409
GenBank Accession LN502768 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K117750-0022-701-G02C-8409
TTACATTGAAGAAGACCGTTCTGACTCTGTCTGTTCAATTTACAGAAGCGACTCCCAAAA
CTATTATGAGAACAATGGGAGTGAAGGGTCTCACTCTCTACCACCTCAAATCACATCTTC
AGGTTTGATAAACAAAACTGATTCCTAATTAACTGTTATTAGATCTATCTTGGTTGATGA
GACCACACCTAGAATTTCAATGACTTGACGCCTTAGATTCTGAAATTTGCTTTGGTATTA
AGAGTTGTATCTAGGTTGGATTTAAGTTTCATTTATATGTGTTATTCATAGTAATATTGG
GTGTTTGATGGTGTTGTGTTTCTCTCTTTTGTATGTGTTTTGCTCGACAGAAATTCCGCC
TAGGGAGGCAAGCTGGCAAAGAATCAACTGAGAACTCTAAAGATGGTATCTATTCAGTTG
ACCTTTTTGTTGTTGTTGTTGTCTATATCTCTGTTTTTGAAGCCGTTTGGTTCTACTCTT
TATTCATATGGCTATTTGGAATCATATTTGTTAGAAGTTTCGTAGAACTATATCATGTGA
GAGGCACAATATATATTTAGTAACTGTTCATGGTTTAAATCGTCAAA

Last Updated on 06.12.2018 16:11