Sequences from confirmation amplicon


Confirmation sequences for line 702E09 and hit At1g20880 are shown.
Line ID 702E09
Locus At1g20880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:7263615
Primers used for PCR 39cs - 8474, show primer details
   
Sequence Name 71-K108745-0022-702-E09C-8409
GenBank Accession LN512576 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>71-K108745-0022-702-E09C-8409
ATTTACATTGAATTATCCTGCAAGTTACCAATCACAACATGTCACATCCTATAGCGTCAC
TCAAGAACCCTAAGTGGCAGAAGATACAACCCCTAACGCAATAGAATCTCTAGATGATTG
TCCATGATGTGATTGGTAAATGCCACTTGATCAAAGTGACTGAAGGACTAGCATGTAGAA
CATTAGAACTGATACACTCATCAAAGCTTGTCTGTTATAGCAGATTTACAATAGCAACGA
AATGTTCTGAGCTTCAAATTGCATAACTCACCCATAAGGGTACACCACTCCTTGTTGGTA
GTTATAAGTAGGGGGTTGGTGATACGGATGGCTTCCAAAATGCAAACCGCGAGGACTCTG
CACACTTCCAACATATGGGGAAGGAGGTCTTACCCGTCCTAACCAAATAAAAGAAGCAAT
GCAGTGATTCAAGGTTAACACGGTCATAATCC
   
Sequence Name 87-K108746-0022-702-E09C-39cs
GenBank Accession LN514777 [GenBank]
Sequencing primer gene specific primer, 39cs: 5'-AGGATTATGACCGTGTTAACCTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>87-K108746-0022-702-E09C-39cs
GACGGGTAGACCTCCTTCCCCATATGTTGGAAGTGTGCAGAGTCCTCGCTAAAGGCATTT
TGGAAGCCATCCGTATCACCAACCCCCTACTTATAACTACCAACAAGGAGTGGTGTACCC
TTATGGGTGAGTTATGCAATTTGAAGCTCAGAACATTTCGTTGCTATTGTAAATCTGCTA
TAACAGACAAGCTTTGATGAGTGTATCAGTTCTAATGTTCTACATGCTAGTCCTTCAGTC
ACTTTGATCAAGTGGCATTTACCAATCACATCATGGACAATCATCTAGAGATTCTATTGC
GTTAGGGGTTGTATCTTCTGCCACTTAGGGTTCTTGAGTGTAGCACTATCATGTGACATG
TTGTGATTGGTAACTTGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCT
ACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAAT
TTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATT

Last Updated on 06.12.2018 16:11