Sequences from confirmation amplicon


Confirmation sequences for line 706F07 and hit At3g26310 are shown.
Line ID 706F07
Locus At3g26310 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:9641887
Primers used for PCR 75we - 8474, show primer details
   
Sequence Name 78-K118393-0022-706-F07C-75we
GenBank Accession LN513745 [GenBank]
Sequencing primer gene specific primer, 75we: 5'-CTTCAGGGTCTTTCCAAGTATCAG-3'
Sequence (A. th genome BLAST matches underlined) 
>78-K118393-0022-706-F07C-75we
CTTGACAGGAATAGTGTAGCCGTTGATATCAAATTCAGACATTGCTTCTCTTGGAAGCAA
AAGAGGTGTTGTAGGATGTAGCCTCCATGTTTCTTTGATCACCATTTTCAGATACTCTAG
CTGATCCATGTCTTCGAAACTTATCATTGATCTGTTCCCCATTTGAGTTCTTATTTCAGA
TTGAACTTTCTTCATCACTCGTGGATTTCTTGCAAGTTCTGTCATTGCCCATGTCATTGT
TATTGCAGAAGTATCGATTCCCGCAAGTAGAACATCCTGTCTCATTCAAAACATATCAGA
CTCTTTGGTACTACTACATTACATAAAGGATTGTTAGCTAAATCTTGTTTTAGTTACCAG
CAAGATTGCTTTGATATGGTTTCTTGTAAGTTTATCATTTCCAAGAACTGCCTCTTCTTT
CTCCAACCTTAAGAGCAAGTCCACAAAATCTACATTCAATTGTAAATGGCTTCATGTCCG
GGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAA
TTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTAT
   
Sequence Name 30-K118392-0022-706-F07C-8409
GenBank Accession LN499915 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K118392-0022-706-F07C-8409
AAGCCATTACATTGAATGTAGATTTTGTGGACTTGCTCTTAAGGTTGGAGAAAGAAGAGG
CAGTTCTTGGAAATGATAAACTTACAAGAAACCATATCAAAGCAATCTTGCTGGTAACTA
AAACAAGATTTAGCTAACAATCCTTTATGTAATGTAGTAGTACCAAAGAGTCTGATATGT
TTTGAATGAGACAGGATGTTCTACTTGCGGGAATCGATACTTCTGCAATAACAATGACAT
GGGCAATGACAGAACTTGCAAGAAATCCACGAGTGATGAAGAAAGTTCAATCTGAAATAA
GAACTCAAATGGGGAACAGATCAATGATAAGTTTCGAAGACATGGATCAGCTAGAGTATC
TGAAAATGGTGATCAAAGAAACATGGAGGCTACATCCTACAACACCTCTTTTGCTTCCAA
GAGAAGCAATGTCTGAATTTGATATCAACGGCTACACTATTCCTGTCAAGACACGGCTTC
ATGTGAATGTTTGGGCTATTGGTCGTGATCCTGATACTTGG

Last Updated on 06.12.2018 16:11