Sequences from confirmation amplicon


Confirmation sequences for line 708C04 and hit At4g14365 are shown.
Line ID 708C04
Locus At4g14365 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:8271692
Primers used for PCR BH62 - 8474, show primer details
   
Sequence Name 16-K104381-0022-708-C04C-BH62
GenBank Accession LN493417 [GenBank]
Sequencing primer gene specific primer, BH62: 5'-TCGTTATTTGTTCATCGGTGTTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K104381-0022-708-C04C-BH62
CTTTTTCACTTTATCATCAACTCTAGTTTTTGTCGGCGTTCGTTCTGTTTTGTATATTTG
GGATAATTCGTAAGTTGTACACNCTGTTTCTTTTCTTTCATATTTGCCAATAAAAAAAAT
CATAGTGTAACAACACGTTCAGTGGAATGCTAGTCCTCTGGAAAAATTGTTAAAGGGTGA
GGTCTTAGATTCGAAAAACTCCAAACATACTAACAAAATGTATAATGGGTCAGAAGGGCT
TATAGGATCGATGCGCTGTAACGCCAACTGAATTCCACGGAACAGGTTGTTACATATAGA
GTTTGTAAGATTGAAGGAAAAATTAACATTGATCATGTAACAAAGATAAAAAGACAAACT
AAACTTGTCGTGATTCTATATTTCGACTTCTTTGACTTCACCACATAACATACACACAAT
AAAAAAATTTGTAGTTATCTTTTAAAGACAAAGTAAAAAATAGGAGATAGTTTCAATATC
TTCCAGCCATAGAGGAGATATGCTTCAAACATGATATAGCTTAATGACCTGATCAATTGT
AAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGG
AGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 32-K104382-0022-708-C04C-8409
GenBank Accession LN500570 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K104382-0022-708-C04C-8409
CCATTTACAATTGATCAGGTCATTAAGCTATATCATGTTTGAAGCATATCTCCTCTATGG
CTGGAAGATATTGAAACTATCTCCTATTTTTTACTTTGTCTTTAAAAGATAACTACAAAT
TTTTTTATTGTGTGTATGTTATGTGGTGAAGTCAAAGAAGTCGAAATATAGAATCACGAC
AAGTTTAGTTTGTCTTTTTATCTTTGTTACATGATCAATGTTAATTTTTCCTTCAATCTT
ACAAACTCTATATGTAACAACCTGTTCCGTGGAATTCAGTTGGCGTTACAGCGCATCGAT
CCTATAAGCCCTTCTGACCCATTATACATTTTGTTAGTATGTTTGGAGTTTTTCGAATCT
AAGACCTCACCCTTTAACAATTTTTCCAGAGGACTAGCATTCCACTGAACGTGTTGTTAC
ACTATGATTTTTTTTATTGGCAAATATGAAAGAAAAGAAACAGGTGTACAACTTACGAAT
TATCCCAAATATACAAAACAGAACGAACGCCGACAAAAACTAGAGTTGATGATAAAAGTA
GAAAAAGTATATTTATTTATATAAACACCGATAAACAAATAAC

Last Updated on 06.12.2018 16:11