Sequences from confirmation amplicon


Confirmation sequences for line 709D04 and hit At2g44270 are shown.
Line ID 709D04
Locus At2g44270 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:18299088
Primers used for PCR HP40 - 8474, show primer details
   
Sequence Name 79-K033554-022-709-D04C-8409
GenBank Accession LN513770 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>79-K033554-022-709-D04C-8409
CCATTTACAATTGATCAATTGGTAGAAGCTAGTCACTGGACATAATGCAGATGATATAGC
AGAAACCGTTCTCTTGAACATATTGCGAGGGGATATTGCTAGGTAAGTATTTGTTGATGA
TTTGATGGAAAATGAAAAATCTGTCTCGTTCCTCAACTAATTGATATTTGTAACATTGGT
AACTGTAGATTAAGTAGGTGCACATCGATTACTACTGGTGAAGATGGTCCCATTCCAAGA
TGTAAACCTTTCAAGTATACATACGAAAAGGAGATTGTCATATATCCTTCACTACATAAT
GCTTTTGTATATATCGCTTATACTTGTCAACAAAAGATATTGTGATACCTTAACGTAGCT
TTACGTATGCTTATTTCAAGAAGCTGGATTACTTCTCCACTGAATGTAAGTGTACAATCA
TCGATTTTTTGTAGGTTTTATCTGTTCCTAAGTTATAGTTTCAGCCAATCTCTAGTTATT
TTTATGGCTTATTTTTTTTTTTTGAGTTTGCAGGCATTTAC
   
Sequence Name 79-K033554-022-709-D04C-HP40
GenBank Accession LN513771 [GenBank]
Sequencing primer gene specific primer, HP40: 5'-ATTAGGAGAGTAAATGCCTGCAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>79-K033554-022-709-D04C-HP40
GCCATAAAAATACTAGAGATTGGCTGAACTATAACTTAGGAACAGATAAAACCTACAAAA
AATCGATGATTGTACACTTACATTCAGGGGAAAAGTAATCCAGCTTCTTGAAATAAGCAT
ACGTAAAGCTACGTTAAGGTATCACAATATCTTTTGTTGACAAGTATAAGCGATATATAC
AAAAGCTTTATGTAGTGAAGGATATATGACAATCCCCTTTTCGTAGGTATACTTGAAAGG
TTTACATCTGGGAAGGGGACCATCTTCACCAGTAGTAATCGATGTGCACCTACTTAATCT
ACAGTTACCAAGGTTACAAATATCAATTATTTGAGGAACGAGACAAATTTTTCATTTTCC
ATCAAATCATCAACAAATACTTACCTAGCAATATCCCCTCGCAATATGTTCAAAAAAACG
GTTTCGGCTATATCATCGGCATTATGTCCAGTGACTAGCTTCTACCAATTTGATCAATTG
TAAAGGGCTTCATGTCCGGGAAATCTACATGGATCACCAATGAGTATGATGGTCAATATG
GAAAAAAAAAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAAATGTCCCCACC
GTTATTATAAAATGAAAGTACTTTTTGATAAAACGACAAATTACAATCCGTCGTATTTAT
AGGCAAAAGCAATAAACAAATTATTCTAATTCGAAAATCTTTATTTCGACGTGTCTACAT
T

Last Updated on 06.12.2018 16:11