Sequences from confirmation amplicon


Confirmation sequences for line 710C11 and hit At2g41220 are shown.
Line ID 710C11
Locus At2g41220 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:17185929
Primers used for PCR 01av - 8474, show primer details
   
Sequence Name 79-K107287-0022-710-C11C-01av
GenBank Accession LN513832 [GenBank]
Sequencing primer gene specific primer, 01av: 5'-CTTATTTCTCCACCTCCACATCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>79-K107287-0022-710-C11C-01av
TGGCCAGTTGATCTTTGATCTCCATCAGGTATTTTACTTTGGCTGTAAATATATTGCCAA
CTTTAGAATCTGGGCTCCCTCTTAACAAAAAATTATAATCAGGTCAACCCCAAGGCAAAA
GTGTCAGTCAAGCTTGTATCAGAAACTGGAATAGGAACAGTTGCCTCCGGAGTAGCGAAG
GCTAATGCCGATATCATACAGGTATGCACATTCTTTCAGAGTTTGTGAGAAATTTGAAAT
TGGAGAATTGTAAATATTTATTCAAATTATACAATTCTGTGGGGGCCATGCTATGGCAAG
ATATTGGCTAATGACGATTCTTATTCCATTTCTCAAAACAATTATGTATATGGTCAAATC
ATGATTCGTATCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGG
ATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTT
TCAATTCAAAAATGTAGATGTCCGCAGCGTTATTA
   
Sequence Name 63-K107286-0022-710-C11C-8409
GenBank Accession LN511003 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K107286-0022-710-C11C-8409
CGGACTGAGCCATTTACATTGAATATATCCTGATACGAATCATGATTTGACCATATACAT
AATTGTTTTGAGAAATGGAATAAGAATCGTCATTAGCCAATATCTTGCCATAGCATGGCC
CCCACAGAATTGTATAATTTGAATAAATATTTACAATTCTCCAATTTCAAATTTCTCACA
AACTCTGAAAGAATGTGCATACCTGTATGATATCGGCATTAGCCTTCGCTACTCCGGAGG
CAACTGTTCCTATTCCAGTTTCTGATACAAGCTTGACTGACACTTTTGCCTTGGGGTTGA
CCTGATTATAATTTTTTGTTAAGAGGGAGCCCAGATTCTAAAGTTGGCAATATATTTACA
GCCAAAGTAAAATACCTGATGGAGATCAAAGATCAACTGTGCCAGATCCTCTATGGAATA
AATATCATGATGTGGAGGTGGAGAAATAAG

Last Updated on 06.12.2018 16:11