Genotyping and primer details


Genotyping and primer details for 710C11 - At2g41220
Line ID 710C11
Locus At2g41220 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:17185929 of line 710C11 Chr2:17185929
   
Insertion allele amplicon 8474 - 01av
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 01av: 5'-CTTATTTCTCCACCTCCACATCAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:17185526
   
wt amplicon (over InsPos) 67dy - 01av
wt amplicon start Chr2:17185526
wt amplicon end Chr2:17186519
wt amplicon size 994 bp
Locus specific primer 01av: 5'-CTTATTTCTCCACCTCCACATCAT-3'
2nd locus specific primer 67dy: 5'-AACCGAATTCAGGGAAATCTTAGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11