Sequences from confirmation amplicon


Confirmation sequences for line 710G07 and hit At2g17390 are shown.
Line ID 710G07
Locus At2g17390 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:7557605
Primers used for PCR SP17 - 8474, show primer details
   
Sequence Name 30-K103556-0022-710-G07C-SP17
GenBank Accession LN499684 [GenBank]
Sequencing primer gene specific primer, SP17: 5'-TGAGTTCACTTTTGCATTTCATTC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K103556-0022-710-G07C-SP17
TACAAATCTCATCTATTAGTATCGTTATCCTCTATGGGCTTTCTACTCTTCATTCTAATT
TACGGTTGGCATGTTAAAAATCTCCAAATTATCTTATCACCGATTTGATTAAACAGTCTA
TTCCTTCTCGAATATAACAGTTTAAGGGTTTTTTCTTTGTGATTGCAACACTAAAGCCCT
GTGGCACACAAAGAAGGACAAAAAAGACTTTAAGAGCCAAAAGAAAAAACCAATTCAAGA
AGAAAATAATTCATTACAAAACCATAATAAACATCCATTTTCTCCATCTTTTTTTTCCAG
ATTTAATGATGAAAACCCCCACAGAAGACAAGGTTGCAATCAGACTCTGTGACACTATTA
CATGACAGAGAAGAAACATATCCTTCAAGAGTCAAAGCAGCTCAGAGGAAAGCGTCCTTC
TCAAGTAGTTTCACCACATCTAGCTGATTGTTTAGCCTGGCCACATCAATGGGATTTTTA
TTGTCCATGTTTTGCTGAGTTCTGCAAACAGAAAATGAAACGACACTTAAAAGAGTCTTA
AAAGAGTCTTATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCA
ATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTC
AAAAATGTAGATATCCGCACGGTTTAT
   
Sequence Name 92-K103557-0022-710-G07C-8409
GenBank Accession LN515191 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>92-K103557-0022-710-G07C-8409
CTTTACAATTGAATATAAGACTCTTTAAGACTCTTTTAAGTGTCGTTTCATTTTCTGTTT
GCAGAACTCAGCAAAACATGGACAATAAAAATCCCATTGATGTGGCCAGGCTAAACAATC
AGCTAGATGTGGTGAAACTACTTGAGAAGGACGCTTTCCTCTGAGCTGCTTTGACTCTTG
AAGGATATGTTTCTTCTCTGTCATGTAATAGTGTCACAGAGTCTGATTGCAACCTTGTCT
TCTGTGGGGGTTTTCATCATTAAATCTGGAAAAAAAAGATGGAGAAAATGGATGTTTATT
ATGGTTTTGTAATGAATTATTTTCTTCTTGAATTGGTTTTTTCTTTTGGCTCTTAAAGTC
TTTTTTGTCCTTCTTTGTGTGCCACAGGGCTTTAGTGTTGCAATCACAAAGAAAAAACCC
TTAAACTGTTATATTCGAGAAGGAATAGACTGTTTAATCAAATCGGTGATAAGATAATTT
GGAGATTTTTAACATGCCAACCGTAAATTAGAATGAAGAGTAGAAAGCCCATAGAGGATA
ACGATACTAATAGATGAGATTTGTACATAAGTAATGTGCAGAATGGAAGTCGATAGAATG
AAATGCAAAAGTGAACTC

Last Updated on 06.12.2018 16:11