Sequences from confirmation amplicon


Confirmation sequences for line 711C08 and hit At5g17780 are shown.
Line ID 711C08
Locus At5g17780 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:5868056
Primers used for PCR JL37 - 8474, show primer details
   
Sequence Name 11-K031934-022-711-C08C-8409
GenBank Accession LN490998 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K031934-022-711-C08C-8409
CTTATGTTCATAATCGAATCTCTTTAAATTAGATTGATGATTAGAAAGCAAACTAATCTC
TCACCGGTGCAACAAGAGTAACGGATTTAACAATGTTGGAATGTTTAGCAGCCAAAGCAA
GAGCAATTAAGCAACCCATTGAATGTGCAACCACATGAAAAGAATCTAATTGGTATGGCT
TAATGACAGATCTCTCTATCGTATCCACATGATCTTTCAATGTATAGAGACTATCCCTTG
GCTTAGGACTCTCCCCGAATCCCAAAAGATCGATCGCGAGGAGCCTATAATCATCTTTTT
GAATGTGTTCAAATACAGTTTCTGTCCAAAAATGTGATGACCCCATAAAACCGTGTATGA
ATATTACATTCTCGGACGGTTCTTGAACAGAGTACTCTGTTTCTTCAAAATGAAACATCA
AAAGCAATTCTAAGTAAAGAACATTATTAACTAATCATAATGCAAAGATTTAATAGAATA
TTAAAATGTTATAATTAATCTATTAACCTCGAGAAGTAGAATCTTTGACCACGACGTGAA
GATTCCCATCTTCGTTCTTAGTCCATGACTTGAAAAGATTT
   
Sequence Name 12-K031934-022-711-C08C-JL37
GenBank Accession LN491457 [GenBank]
Sequencing primer gene specific primer, JL37: 5'-CAAATCTTGCAAGTCATGGACTAA-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K031934-022-711-C08C-JL37
CGTGGTCAAGATTCTACTTCTCGAGGTTAATAGATTAATTATAACATTTTAATATTCTAT
TAAATCTTTGCATTATGATTAGTTAATAATGTTCTTTACTTAGAATTGCTTTTGATGTTT
CATTTTGAAGAAACAGAGTACTCTGTTCAAGAACCGTCCGAGAATGTAATATTCATACAC
GGTTTTATGGGGTCATCACATTTTTGGACAGAAACTGTATTTGAACACATTCAAAAAGAT
GATTATAGGCTCCTCGCGATCGATCTTTTGGGATTCGGGGAGAGTCCTAAGCCAAGGGAT
AGTCTCTATACATTGAAAGATCATGTGGATACGATAGAGAGATCTGTCATTAAGCCATAC
CAATTAGATTCTTTTCATGTGGTTGCACATTCAATGGGTTGCTTAATTGCTCTTGCTTTG
GCTGCTAAACATTCCAACATTGTTAAATCCGTTACTCTTGTTGCACCGGTGAGAGATTAG
TTTGCTTTCTAATCATCAATCTAATTTTAAAGAGATTCGATTATGAACATAAGTTCGTGT
CCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAG
TAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGA
AAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAA
ACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCC
Primers used for PCR FQ52 - 8474, show primer details
   
Sequence Name 37-K035336-022-711-C08C-8409
GenBank Accession LN502525 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K035336-022-711-C08C-8409
GCCATTTGTATTGTTTTTCTTTATCAATGCAGCCTTATTTTCCTTCATCGGTAGAAGGAT
CGGTCTTGAACCGAATCGCTAGAAAGCGGTTGTGGCCACCGCTTGCGTTTGGGACAGCGG
TGATGTCTTGGTATGAACATATTGGTAGATGCGTATGTTTCATAATCTGCAAGCATCATA
AGATATGGGAGTGGCTAATCAAGCTATGTATAGGTAAAAGGTAAAAACATTTGAACCGGT
TTGGGTGATTGTTTCAG
   
Sequence Name 37-K035336-022-711-C08C-FQ52
GenBank Accession LN502526 [GenBank]
Sequencing primer gene specific primer, FQ52: 5'-AAAAACTGAAACAATCACCCAAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K035336-022-711-C08C-FQ52
TTTTACCTATACTAGCTTGATTAGCCACTCCCATATCTTATGATGCTTGCAGATTATGAA
ACATACGCATCTACCAATATGTTCATACCAAGACATCACCGCTGTCCCAAACGCAAGCGG
TGGCCACAACCGCTTTCTAGCGATTCGGTTCAAGACCGATCCTTCTACCGATGAAGGAAA
ATAAGGCTGCATTGATAAAGAAAAACAATACAAATGGCTTCATGTCCGGGAAATCTACAT
GGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTT
TTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATA
AAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAAT
TCGGAAATCTTTATTTCGACGTGTCTACATTCCCGTCCAAATGGG

Last Updated on 06.12.2018 16:11