Sequences from confirmation amplicon


Confirmation sequences for line 711G12 and hit At1g26460 are shown.
Line ID 711G12
Locus At1g26460 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:9152307
Primers used for PCR 03bo - 8474, show primer details
   
Sequence Name 04-K107640-0022-711-G12C-03bo
GenBank Accession LN487947 [GenBank]
Sequencing primer gene specific primer, 03bo: 5'-GGCTACACAACTACGAACACATTC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K107640-0022-711-G12C-03bo
CCAGATTTCGTGCCGTGTCCTAACTTTCATGGCTTCGTCATTCTTCCCAACTCCAAAATG
CATTCCAATAACCAAATCATATGATTCATCATCCGGAAGAGAGTCCTTCCCCAAGAGGAG
CATCCTGACCAAATTACATCAAGTCATGATACATTTTGAAAAGATCTACATTTACATGAC
AACAGTATCAGTAGCTGCTATGCAGAGATAACCTTTGATTCATCAGCAAATCTTACCGTT
CAATCATGTAAAGTCTTAATCTTTACACTATTTCTAGAAGCATTGTTCATTGTACAAAAA
AGGAATCTATCTACGAACTCAGAGAATCTAACCGTTCAAGCAGCTTCATGGCGGCTTCAG
TCTCTCTTGCCTGGTACATAGCCTTCAAAACTAGATTATAAGACGCTGTATTAGGTTCAA
CAGAAAACTCCTCCATTGGCGCAACAAGATCAAGCATATCTCCAGCAGAAGCACCCATCA
TCAAGTTAATGTAAATGGCTTATTCGGCTTATATATTCAATTGTAAATGGCTTCATGTCC
GGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTTATT
   
Sequence Name 20-K107641-0022-711-G12C-8409
GenBank Accession LN495241 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K107641-0022-711-G12C-8409
TCGGACATGAGCCATTTACAATTGAATATATAAGCCGAATAAGCCATTTACATTAACTTG
ATGATGGGTGCTTCTGCTGGAGATATGCTTGATCTTGTTGCGCCAATGGAGGAGTTTTCT
GTTGAACCTAATACAGCGTCTTATAATCTAGTTTTGAAGGCTATGTACCAGGCAAGAGAG
ACTGAAGCCGCCATGAAGCTGCTTGAACGGTTAGATTCTCTGAGTTCGTAGATAGATTCC
TTTTTTGTACAATGAACAATGCTTCTAGAAATAGTGTAAAGATTAAGACTTTACATGATT
GAACGGTAAGATTTGCTGATGAATCAAAGGTTATCTCTGCATAGCAGCTACTGATACTGT
TGTCATGTAAATGTAGATCTTTTCAAAATGTATCATGACTTGATGTAATTTGGTCAGGAT
GCTCCTCTTGGGGAAGGACTCTCTTCCGGATGATGAATCATATGATTTGGTTATTGGAAT
GCATTTTGGAGTTGGGAAGAATGACGAAGCCATGAAAGTTATGGACACGGCACTGAAATC
TGGTTATATGTTATCCACCAGTGTCTTTACTGAATGTGTTCGTAGTGGTGTAGC

Last Updated on 06.12.2018 16:11