Sequences from confirmation amplicon


Confirmation sequences for line 713F07 and hit At3g16540 are shown.
Line ID 713F07
Locus At3g16540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:5628269
Primers used for PCR CP21 - 8474, show primer details
   
Sequence Name 58-K031139-022-713-F07C-CP21
GenBank Accession LN509606 [GenBank]
Sequencing primer gene specific primer, CP21: 5'-AACGACTTTGTTTCCCATAATCAC-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K031139-022-713-F07C-CP21
TGTATTGATAGCAGATCACTGTCACTGTAATCGTATATTCTAGTTTCAACTCTAAGTACT
AGACCTTTTGTAATGCAGATATTTTCACCACCTGCATTTTGAGCACATTAATATAATTCT
ACTAACCAAATCATAAGAAAAATTTCATCATTTAGCATAATTTTATGGTATATTGGAATA
TGAATTATAAGATTGTAGGAAACATGCATGATATGTCTCCATATGATTTATTACCAACAA
CGGACACAACTTCTTGGAGAGGTGGTATGTCTCCAAGCTCCAAGGGATTCATACCCTTCC
AAAACTCGTCACTATCGATCTCCAAGATGGCCAAATCACATTCATGTGAAATCTTTTGAA
CTTTTGCTTTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGA
TGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAG
ATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCC
GTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGA
CGTGTCTACATTCAC
   
Sequence Name 23-K031139-022-713-F07C-8409
GenBank Accession LN496441 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K031139-022-713-F07C-8409
ATTTCAAGCAAAGTTCAAAAGATTTCACATGAATGTGATTTGGCCATCTTGGAGATCGAT
AGTGACGAGTTTTGGAAGGGTATGAATCCCTTGGAGCTTGGAGACATACCACCTCTCCAA
GAAGTTGTGTCCGTTGTTGGTAATAAATCATATGGAGACATATCATGCATGTTTCCTACA
ATCTTATAATTCATATTCCAATATACCATAAAATTATGCTAAATGATGAAATTTTTCTTA
TGATTTGGTTAGTAGAATTATATTAATGTGCTCAAAATGCAGGTGGTGAAAATATCTGCA
TTACAAAAGGTCTAGTACTTAGAGTTGAAACTAGAATATACGATTACAGTGACAGTGATC
TGCTATCAATACAGATTGATGCAACTATCAACGATGAAAATAGTGGTGGCCCGGTGATTA
TGGGAAACAAAAGTCGT

Last Updated on 06.12.2018 16:11