Sequences from confirmation amplicon


Confirmation sequences for line 714B08 and hit At1g07420 are shown.
Line ID 714B08
Locus At1g07420 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:2279932
Primers used for PCR CR42 - 8474, show primer details
   
Sequence Name 30-K107199-0022-714-B08C-CR42
GenBank Accession LN499744 [GenBank]
Sequencing primer gene specific primer, CR42: 5'-ATTTGGTAGCGCAACATCTAAACT-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K107199-0022-714-B08C-CR42
AGCTTCAATAGATGACCCATACAAAATGTTTTAATTGCCCAACCTAAACACATACTGAGT
TGAAGTACCATATGTTTTTTCAAATAGGCCACACGAGCAGCAATTCTAGTCAGACATAAT
AAACTAAGACACATGAGCGTTGCAGCAACCTGAAAGCAAAATGTTAAAGAAAAGGAGAGG
TTGCAGAATAAGACCTCTCTAAGACAAATGTACTCTGCAATCCAAAGTGGCAATTTCACG
TTTGTTTCATGTCACCGTTTTCTTTAAGGGTCTTCAGTCTTCTGTACCCCTTGTCAGTAC
CAAAGATCCTGAAGAACACCAGAGACGAATTAATACACAAAAATCTACAATAGTAATTAC
CAATGATAATTACCCATGTATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACAT
GGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTT
TTTCAATTCAAAAATGTAGATGTCCGCACGNTTATT
   
Sequence Name 14-K107198-0022-714-B08C-8409
GenBank Accession LN492533 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K107198-0022-714-B08C-8409
CGGAATGAGCCTTTACAATTGAATATACATGGGTAATTATCATTGGTAATTACTATTGTA
GATTTTTGTGTATTAATTCGTCTCTGGTGTTCTTCAGGATCTTTGGTACTGACAAGGGGT
ACAGAAGACTGAAGACCCTTAAAGAAAACGGTGACATGAAACAAACGTGAAATTGCCACT
TTGGATTGCAGAGTACATTTGTCTTAGAGAGGTCTTATTCTGCAACCTCTCCTTTTCTTT
AACATTTTGCTTTCAGGTTGCTGCAACGCTCATGTGTCTTAGTTTATTATGTCTGACTAG
AATTGCTGCTCGTGTGGCCTATTTGAAAAAACATATGGTACTTCAACTCAGTATGTGTTT
AGGTTGGGCAATTAAAACATTTTGTATGGGTCATCTATTTGAAGCTTAATATATCCTTGT
CTCATTTTTCTGTATCAAGTTTAGATGTTGCGCTACCAAATA

Last Updated on 06.12.2018 16:11