Sequences from confirmation amplicon


Confirmation sequences for line 714G06 and hit At2g29880 are shown.
Line ID 714G06
Locus At2g29880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:12742892
Primers used for PCR 33vh - 8474, show primer details
   
Sequence Name 65-K117914-0022-714-G06C-33vh
GenBank Accession LN511485 [GenBank]
Sequencing primer gene specific primer, 33vh: 5'-TGTCATGGTCAGATCAAGAATGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K117914-0022-714-G06C-33vh
CAACGAGGCTGGCGTGACAAAATGGTACCATAAGCAAAACTACGGTAGAGAGAAAAATAC
TACCTCTTCTAAATAAAAAGTTTAAATGTAATAAGACTTACACAAATTATTTAAGTCGAA
TGAAGTCTATGAAAAAGGAGTATTCTGTGTATGCTGCACTTTTTTGGTTTAGTTCTGGTT
TTGGATGGGATCCCATCACAAAACAATTTACAGCTCCAGATGATGTGTGGGCAGCCTATT
TGTGTGGGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATC
AGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCA
ATTCAAAAATGTAGATGTCC
   
Sequence Name 25-K117913-0022-714-G06C-8409
GenBank Accession LN497646 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K117913-0022-714-G06C-8409
GCATTTCATTGAATAATCCTGCCCACCAAATAGGCTGCCCACACATCATCTGGAGCTGTA
AATTGTTTTGTGATGGGATCCCATCCAAAACCAGAACTAAACCAAAAAAGTGCAGCATAC
ACAGAATACTCCTTTTTCATAGACTTCATTCGACTTAAATAATTTGTGTAAGTCTTATGA
CATTTAAACTTTTTATTTAGAAGAGGTAGTATTTTTCTCTCTACCGTAGTTTTGCTTATG
GTACCATTTTTGTCACGCCAGCCTCGTTTGAGAGCATCAACTAAGATAGCCGTTAACTCA
TAACATTCTTGATCTGACCATGACAACANAAAAGATTGAGTACTTACCATTTTTTTTTCA
ATTCAGAAAAGTACATGTCCGCA

Last Updated on 06.12.2018 16:11