Sequences from confirmation amplicon


Confirmation sequences for line 715D10 and hit At4g02600 are shown.
Line ID 715D10
Locus At4g02600 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:1146978
Primers used for PCR RP14 - 8474, show primer details
   
Sequence Name 73-K027490-022-715-D10C-8409
GenBank Accession LN512814 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K027490-022-715-D10C-8409
AGCCATTTACATTGCTTTTAGGTCTCTCTTTTGTTTCACTTTCTGTGCCCTACCAACAAG
ACCAACCTGCACATTCTCCTCGAATATAGCTTTCTTGAAGCTACTTCCCATCTGTTGTAA
TATAAGAAAAGGGTCAACTTTTCAGAAAGAATCCAAAAAACCAAACCAAAAGATTCTCAA
GCTTTTTAGTAAAAATTAGCTTGTTACCTGTGAGACGATGGCGTAAAGAGGCAGTGTACT
GTAACTGCAAAGCACTTGAATGAAGACCCTAAAGAGAAGAAGAAAGATACCAGTCTACAA
TGAGTTATACTCAACGAAACAATATGAGAAAGAACAAAAGAAAAAATGTCGAATCTTTAT
GCTTACCCGATAACCAATCTTGGAACAATGTATCTCACCTGTCCCATAATGCACGAGTCG
AAGCCGTATGTAACCTAAAATAAAGTCATCAGTCTTAACCATTTTAGGTGAATCTTAAAA
TTTTGTTTTTTCTTTTCTTAAAGAACCAGACTTACCCAAATCCAAAAGAAAAACGCAATC
TCAAAAGCATTCTGGAAGAGGATAAAATGGATCAAGTAGAGAACAATTTGAGGTTTGCTG
AACCAGAAATGCTCATCTGAGGGT
   
Sequence Name 74-K027490-022-715-D10C-RP14
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, RP14: 5'-AGACTTAGTGGTGAAACCCTCAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>74-K027490-022-715-D10C-RP14
GCAGGGGAATCTGCGTCATTGTTCGTCTTGCGAGACAAATTCAC

Last Updated on 06.12.2018 16:11