Sequences from confirmation amplicon


Confirmation sequences for line 717A02 and hit At5g06870 are shown.
Line ID 717A02
Locus At5g06870 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:2134061
Primers used for PCR 30mm - 8474, show primer details
   
Sequence Name 28-K114505-0022-717-A02C-30mm
GenBank Accession LN498956 [GenBank]
Sequencing primer gene specific primer, 30mm: 5'-CGAGTTTACGTAATGAAGAGAGGG-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K114505-0022-717-A02C-30mm
TCATGTACTCAAGATTCTTGAGCTGACTCAGAAACTCAGGAACCGGACCGGTGAGATTGG
TCCAGCTTAGTCTGAGAAAAGTGAGGTTCTTGAGCTTGGCGATAGTGGGTTGGATGTGAC
CAGTGAGGTTAGTGAGTTTGCGGAAGATAAGGGATGTAAGATACGGTAAGTCACCGACTT
CAGGGGGGATCTGACCGGAGATCTCGCCGTCTTGTATGATTAGGGAAGTGACACGGTGGT
TGACAGTAGCGTCGCCGCACTCGAGGCAGTACCAAGAGCAACAGTCGGTTTTGGGATCCC
ATGAGGCGAGGTGGTAAGGGTTGTTTATGGACAGGATATATTCAATTGTAAATGGCTTCA
TGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAA
GAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCA
   
Sequence Name 03-K114504-0022-717-A02C-8409
GenBank Accession LN487585 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K114504-0022-717-A02C-8409
GCATTTCATTGAATATATCCTGTCCATAACAACCCTTACCACCTCGCCTCATGGGATCCC
AAAACCGACTGTTGCTCTTGGTACTGCCTCGAGTGCGGCGACGCTACTGTCAACCACCGT
GTCACTTCCCTAATCATACAAGACGGCGAGATCTCCGGTCAGATCCCCCCTGAAGTCGGT
GACTTACCGTATCTTACATCCCTTATCTTCCGCAAACTCACTAACCTCACTGGTCACATC
CAACCCACTATCGCCAAGCTCAAGAACCTCACTTTTCTCAGACTAAGCTGGACCAATCTC
ACCGGTCCGGTTCCTGAGTTTCTGAGTCAGCTCAAGAATCTTGAGTACATTGACCTTTCC
TTCAATGACCTCTCTGGTTCCATACCTAGTTCCCTCTCTTCATTACNTAAACTC

Last Updated on 06.12.2018 16:11