Sequences from confirmation amplicon


Confirmation sequences for line 718G01 and hit At5g05370 are shown.
Line ID 718G01
Locus At5g05370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:1591474
Primers used for PCR 68ra - 8474, show primer details
   
Sequence Name 45-K115914-0022-718-G01C-68ra
GenBank Accession LN505645 [GenBank]
Sequencing primer gene specific primer, 68ra: 5'-TTGTGACGGAGAGATGCATATTAG-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K115914-0022-718-G01C-68ra
AATCGGAGAATTGGTCGTCGAAATGGGAAAGCAGCCGGTGAAATTGAAGGCGGTGGTTTA
CGCGTTATCGCCGTTTCAGCAGAAGATCATGACGGGTCTTTGGAAGGATCTTCCGGAGAA
GATTCACCACAAGGTCTCAGAGAATTGGATCAGTACTATTCTCCTCGTCGCTCCTGTCGT
CGGAACCTACTCGTACGTTTTTCTCTTTCTCTCGGTCTAGGCAATTAACAAACAGATTTG
ATGTATCAATCATGTGGACTTCGATCTGATGATTATGATGTTCAACAATCTGATTGTTAT
GCGAAGTTTGTAGATTCAATTAGGCTATAGTTTTATTGGATATGACAGCAAAAATTGTTT
TAGGGCAATTGTTCTGTGACAAATTCGCATAGATTGGTGTCGATTTCGATGCTCATGTTG
TAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATG
GAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 19-K115913-0022-718-G01C-8409
GenBank Accession LN494905 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K115913-0022-718-G01C-8409
ATTACACATGAGCATCGAAATCGACACCAATCTATGCGAATTTGTCACAGAACAATTGCC
CTAAAACAATTTTTGCTGTCATATCCAATAAAACTATAGCCTAATTGAATCTACAAACTT
CGCATAACAATCAGATTGTTGAACATCATAATCATCAGATCGAAGTCCACATGATTGATA
CATCAAATCTGTTTGTTAATTGCCTAGACCGAGAGAAAGAGAAAAACGTACGAGTAGGTT
CCGACGACAGGAGCGACGAGGAGAATAGTACTGATCCAATTCTCTGAGACCTTGTGGTGA
ATCTTCTCCGGAAGATCCTTCCAAAGACCCGTCATGATCTTCTGCTGAAACGGCGATAAC
GCGTAAACCACCGCCTTCAATTTCACCGGCTGCTTTCCCATTTTCGACGACCAAATCTCC
GATTTCGATAAATGCAACTAGGAGACTTTTCAAGCCGGCTAATATGCA

Last Updated on 06.12.2018 16:11