Sequences from confirmation amplicon


Confirmation sequences for line 718H06 and hit At1g60800 are shown.
Line ID 718H06
Locus At1g60800 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:22384565
Primers used for PCR KH79 - 8474, show primer details
   
Sequence Name 75-K103202-0022-718-H06C-8409
GenBank Accession LN513224 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K103202-0022-718-H06C-8409
CTTTACATTGAATATATCCTGACATGATATCAAAATGGGTCACACTTGCTCGTGTAGGTA
AACTAGTCCTCTCGCTGTCCCAACCGCTATCTTCTTCCTTCTCGACCAGTCTAATGCTGG
CTCTCCACGGATATTATCTAAACAAAAAATGTAGCAAGATGGAACTATTAAAAACTTGCA
AAAGAGCATGAAGAAATTAGCAGTTTTTTTGGAAACTGCATTACCTTTTAAGCGTGATGC
GACACTCCCATTTGGCATGTAAGGGTAGACTAAAATTCTCTCCTGGTTGCTACTACAGAA
ACCGCGGAGCCGGAGGAGATTGCGATGAAGAGCCAAACTTATAGTCTCTACTTCTGTCTG
AAACTGGACTTCTCCACCCGCAATGTTACAGTCCTTGAGACGTTTGACAGCCACCAAAGT
TCCATCGTTTAAGTGTCCTTTGTACACAATCCCGTATCCGCCTCTTCCGAGAATGTTCTT
CGAGTTGAAATGATTGGTGGCAGATCTNAGCTCTTTGAATGTATACCTCTTCAAGTGCCC
TAAACTCACTTCTGGATCATATTGTTCTGCANAATATTAATCATCANGATGCANGTGTCA
GTTTTGCTCTGCACAAAATGATGTGAAGCCAATNGTATTTCANGATACTAACCATTAACG
TCAAAAAATATTTGCTTGTTACGGCAATNTCTCCACCAAAGAAACATTCCGCTTGTAAAG
AANACAAAAAATGCTGCACTGAAGCTGGCGGCAAATGCAAGAGCAACGTGNTGGCCATTG
GTACGAGTTCCTGATTCATC
   
Sequence Name 76-K103202-0022-718-H06C-KH79
GenBank Accession LN513380 [GenBank]
Sequencing primer gene specific primer, KH79: 5'-CACTTCCATCTTTGGTTCTTCTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>76-K103202-0022-718-H06C-KH79
GGCCATCACGTTGCTCTTGCATTTGCCGCAAGCTTCAGTGCAGCATTTTTTGTTTTCTTT
ACAAGCGGAATGTTTCTTTGGTGGAGATATCGCCGTAACAAGCAAATATTTTTTGACGTT
AATGGTTAGTATCTTGAAATACTATCGGCTTCACATCATTTTGTGCAGAGCAAAACTGAC
ACTTGCATCTTGATGATTAATATTTTGCAGAACAATATGATCCAGAAGTGAGTTTAGGGC
ACTTGAAGAGGTATACATTCAAAGAGCTTAGATCTGCCACCAATCATTTCAACTCGAAGA
ACATTCTCGGAAGAGGCGGATACGGGATTGTGTACAAAGGACACTTAAACGATGGAACTT
TGGTGGCTGTCAAACGTCTCANGGACTGTAACATTGCGGGTGGAGAAGTCCAGTTTCAGA
CAGAAGTAGAGACTATNAGTTTGGCTCTTCATCGCAATCTCCTCCGGCTCCGCGGTTTCT
GTAGTAGCAACCAGGAGAGAATTTTAGTCTACCCTTACATGCCAAATGGGAGTGTCGCAT
CACGCTNAANAGGTAATGCAGTTTCCAAAAAAACTGCTAATTTCTTCATGCTCTTTTGCA
AGTTTTTAATAGTTCCATCTTGCTACATTTTTTGTTTAGATAATATCCGTGGAGAGCCAG
CATTAGACTGGTCGAGAAGGAAGAAGATAGCGGTTGGGACAGCGAGAGGACTAGTTTACC
TACACGAGCAATGTGACCCATTTTGATATCATGTCAGGATATATTCAATTGTAAATGGCT
TCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAANAAG
AANGAGTAATTACCAATTTTTTTTCATTTCANAAATGTA

Last Updated on 06.12.2018 16:11