Sequences from confirmation amplicon


Confirmation sequences for line 721A10 and hit At1g51170 are shown.
Line ID 721A10
Locus At1g51170 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:18953725
Primers used for PCR AB65 - 8474, show primer details
   
Sequence Name 44-K105566-0022-721-A10C-AB65
GenBank Accession LN505185 [GenBank]
Sequencing primer gene specific primer, AB65: 5'-AAGAGATACTCCACTGTCCCTTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K105566-0022-721-A10C-AB65
CGTAGTAAACTCGTAATCATCAAGTACCATGCTTTACGGAACAATTAGACCGTGTGGGTT
ATATGAATCAACAGTCCTTTTACTATTTTCCTCTTAGTTATCAGGATCAATTATTTCCCC
TAGTAACGTTGTTTAAACTTTTGTTGATACTGGTGACGTATATATATCTATCACAACGTA
TTTTTCTTCAGCTAATTTGTTGGCTTCCGTCAGTTATCTATAACAATAATTTGTAACAAT
ACTAGTTTAAAGTTTATTATATTTTCGGTTTCTTGATTTTTGTTCTAACAATTATTTGTT
TATACACATCAAAATCAGAAAAGCTAACAGAACAAGCAAAAAAAAAAAAAAATCTAAAAA
ACTATTATACGTGTGATAAATTCTCCCACGCGGGGATCAAAAATCAACAAACGGATTGTT
TTCAAAACACTCGTGCGGTAACGGTAACGGCGGCTTTTTCAGTTTCTCAAAATATTCCTT
TATACCAAACCCACTTTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACTTGGATCA
CCAATGAGTATGATGGTCAATATGGAAAAAAAAAAAAAGTAATTACCAATTTTTTTTCAT
TTCAAAATTGTA
   
Sequence Name 76-K105567w-0022-721-A10C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>76-K105567w-0022-721-A10C-8409
CATTTACATTGAAAAGTGGGTTTGGTATAAAGGAATATTTCGAGAAACTGAAAACGCCGC
CGTTACCGTTACCGCACGAGTGTTCTGAAAACAATCCGTTTGTTGATTTCTGATCCACGC
GTGGGAGAATCTATCACACGTATAATAGTTTTTTAGATTCTCTTTTTTTTTTGCTTGTTC
GGTTACCTTTTCGGATTTTGATGTGTATAAACAAATAATTGTTAGAACAAAAATCAAGAA
ACCGAAAATATAATAAACTTTAAACTAGTATTGTTACAAATTATTGTTATAGATAACTGA
CGGAAGCCAACAAATTACCTGAAAAAAAATACTTTGTGATAGATATATATACGTCACCAG
TATCAACAAAAGTTTAAACAACGTTACTAGGGGAAATAATTGATCCTGATAACTAAAAGG
AAAATAGTAAAGGGACTGTTGATTCATATAACCCACACGGTCTAATTGTTCCGTAAAGCA
GGGTACTTGATGATTACAATTTTACTACGATGTTTAAACGACCCACCGAAGGTCATTTGT
GTTATTGCAAAGGGA

Last Updated on 06.12.2018 16:11