Sequences from confirmation amplicon


Confirmation sequences for line 722C11 and hit At3g55140 are shown.
Line ID 722C11
Locus At3g55140 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:20439836
Primers used for PCR 55sm - 8474, show primer details
   
Sequence Name 50-K116491-0022-722-C11C-55sm
GenBank Accession LN507291 [GenBank]
Sequencing primer gene specific primer, 55sm: 5'-GGTCATGATGTTGATGGTATTCAG-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K116491-0022-722-C11C-55sm
TTTGGATTATCGTGTGTCTCCGCGATACGATGATGGGCTTATTGATATTACTAGACAAAG
CACGGATATTACTGTTTCTAGGTGAGGTTTGATGATTTTGGATGGTTATGGTTAGGGTAA
TGTATGCTCTTGACTGGTGCTTAATGTTGCTTCTGGTTTCTACAGATGTTATTTTGCACA
GCATGACAAGACGATGCTGATTGGAGCAGATCCTTCTCATGTTGAGGATAGATGTATCCG
AGTGACCATTCACCATTGTTTCTTTGATGGAACTAGGCAGAGACAACCTCGTCTCAGATT
TGGAAAAGTTCATTTGTACAACAACTACACAAGAAACTGGGGAATATATGCTGTTTGTGC
TAGTGTAGAAGCGCAGGTAAACTCTTTTGCAGATCTTAGATCATTGCTTGAGGTCTCACC
TGAAGATTCTTATGTTTCTGAAATCAGGTGTTCTCTCAATGGCTTCATGTCCGGGAAATC
TACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCAAAAATGTAGATAATCCACACGTTATT
   
Sequence Name 18-K116490-0022-722-C11C-8409
GenBank Accession LN494465 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K116490-0022-722-C11C-8409
GAGACACCTGATTTCGAAACTAAGAATCTTCAGGTGAGACCTCAAGCAATGATCTAAGAT
CTGCAAAAGAGTTTACCTGCGCTTCTACACTAGCACAAACAGCATATATTCCCCAGTTTC
TTGTGTAGTTGTTGTACAAATGAACTTTTCCAAATCTGAGACGAGGTTGTCTCTGCCTAG
TTCCATCAAAGAAACAATGGTGAATGGTCACTCGGATACATCTATCCTCAACATGAGAAG
GATCTGCTCCAATCAGCATCGTCTTGTCATGCTGTGCAAAATAACATCTGTAGAAACCAG
AAGCAACATTAAGCACCAGTCAAGAGCATACATTACCCTAACCATAACCATCCAAAATCA
TCAAACCTCACCTAGAAACAGTAATATCCGTGCTTTGTCTAGTAATATCAATAAGCCCAT
CATCGTAATCGCGGAGACTACACCGATCAATCCAAATGTGTCTAGACTTTGGTTTAATCT
GAATACCATCAACTTCATGACC

Last Updated on 06.12.2018 16:11