Sequences from confirmation amplicon


Confirmation sequences for line 722F09 and hit At4g33330 are shown.
Line ID 722F09
Locus At4g33330 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:16060974
Primers used for PCR DF03 - 8474, show primer details
   
Sequence Name 33-K029125-022-722-F09C-8409
GenBank Accession LN500871 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K029125-022-722-F09C-8409
TAGATTTCCCGGACATGAAGCCATTTTTCTCGCCGCCGCAGGATGGAAGCTTCGTCGGAT
CATTCGAATCAGAAACCCACTTGCGGAGAAGGACTCGTACAATGAATACAACTACAGCAA
GTTTCGACTCTGGCAATTGACAGATTACGACAAAGTGATCTTCATTGATGCCGACATCAT
CGTCTTACGTAACCTTGATCTTCTCTTCCATTTTCCTCAGATGTCGGCCACCGGAAATGA
TGTATGGATATATAATTCAGGCATCATGGTCATCGAGCCTTCTAATTGTACGTTTACTAC
AATCATGAGCCAGCGAAGCGAGATCGTTTCATACAACGGTGGAGATCAAGGGTGATTAAT
TAGCACTTTTAGTATTACATTATAGACTTTATACTTAACAATTATATTAACAAAAAAAGA
AAATATATATTTTCAGGTACCTAAACGAGATATTTGTGTGGTGGCACCGATTGCCTCGAC
GAGTAAACTTTCTGAAGAACTTCTGGTCGAACACAACCAAAGAAAGAAACATCAAGAACA
ACCTCTTCGCCGCGGAGCCGCCTCAGGTCTACGCGGTCCACTACTTAGGTTGGAAACCAT
GGCTTNTGCTATA
   
Sequence Name 34-K029125-022-722-F09C-DF03
GenBank Accession LN501288 [GenBank]
Sequencing primer gene specific primer, DF03: 5'-CAATCGTAGTCCCTATAGCAAAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K029125-022-722-F09C-DF03
CATGGTTTCCAACCTAAGTAGTGGACCGCGTAGACCTGAGGCGGCTCCGCGGCGAAGAGG
TTGTTCTTGATGTTTCTTTCTTTGGTTGTGTTCGACCAGAAGTTCTTCAGAAAGTTTACT
CGTCGAGGCAATCGGTGCCACCACACAAATATCTCGTTTAGGTACCTGAAAATATATATT
TTCTTTTTTTGTTAATATAATTGTTAAGTATAAAGTCTATAATGTAATACTAAAAGTGCT
AATTAATCACCCTTGATCTCCACCGTTGTATGAAACGATCTCGCTTCGCTGGCTCATGAT
TGTAGTAAACGTACAATTAGAAGGCTCGATGACCATGATGCCTGAATTATATATCCATAC
ATCATTTCCGGTGGCCGACATCTGAGGAAAATGGAAGAGAAGATCAAGGTTACGTAAGAC
GATGATGTCGGCATCAATGAAGATCACTTTGTCGTAATCTGTCAATTGCCAGAGTCGAAA
CTTGCTGTAGTTGTATTCATTGTACGAGTCCTTCTCCGCAAGTGGGTTTCTGATTCGAAT
GATCCGACGAAGCTTCCATCCTGCGGCGGCGAGAAAAATGGCTTCATGTCCGGGAAATCT
ACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAAT
TTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTT
GATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTC
TAATT

Last Updated on 06.12.2018 16:11