Genotyping and primer details


Genotyping and primer details for 722F09 - At4g33330
Line ID 722F09
Locus At4g33330 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:16060974 of line 722F09 Chr4:16060974
   
Insertion allele amplicon 8474 - DF03
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer DF03: 5'-CAATCGTAGTCCCTATAGCAAAGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:16060377
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11