Sequences from confirmation amplicon


Confirmation sequences for line 722H01 and hit At2g24360 are shown.
Line ID 722H01
Locus At2g24360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:10365806
Primers used for PCR 35tg - 8474, show primer details
   
Sequence Name 38-K116785-0022-722-H01C-35tg
GenBank Accession LN503123 [GenBank]
Sequencing primer gene specific primer, 35tg: 5'-TGCTGAGATGAGAAGGTTATCTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K116785-0022-722-H01C-35tg
ACCCCTAGCAACATCCAAGCCTGTTTAACAGCTAACTTCAAAGGGACGGCTCGGTTCTGT
CTCCTAGTCAAAAACTGCCTCACTGAACCTCCTTTGGCGTATTCAGTCACTATACACCAC
ACCATTGGCTTGCGACATGCACCAATGAACCTCACAATGTTTGGGTGCTTCAAATTAGCA
AGCATAGACACCTCTTGCTGAAACTGTTGTTCCATGAACTGTGCCTTTTCTGGGCTGTTC
TCTGGCCGCTCAAGTATTTTGATAGCTACATCTTCACCGTTGTATGTCCCTTTGTATAAT
TTACCAAAAGCCCCTTGAGCAAAGGCAGGACCCATGTTGAGTTTCCTCAGATCAATCGTC
CACTCATCATAGTTCGTCAGCCCTTCAGTTGGATACCTGGTATCCATCAGTGCTTGAGCC
AAAGCATCATCATTCAACGCATGGGTAACTCTTCCCGGGAAATGGCTTCAATGTCCATAT
ATTAACAAATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCA
ATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTC
AAAAAT
   
Sequence Name 19-K116784-0022-722-H01C-oriL
GenBank Accession LN494926 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K116784-0022-722-H01C-oriL
TTTGTTAATATATGGACATTGAAGCCATTTCCCGGGAAGAGTTACCCATGCGTTGAATGA
TGATGCTTTGGCTCAAGCACTGATGGATACCAGGTATCCAACTGAAGGGCTGACGAACTA
TGATGAGTGGACGATTGATCTGAGGAAACTCAACATGGGTCCTGCCTTTGCTCAAGGGGC
TTTTGGTAAATTATACAAAGGGACATACAACGGTGAAGATGTAGCTATCAAAATACTTGA
GCGGCCAGAGAACAGCCCAGAAAAGGCACAGTTCATGGAACAACAGTTTCAGCAAGAGGT
GTCTATGCTTGCTAATTTGAAGCACCCAAACATTGTGAGGTTCATTGGTGCATGTCGCAA
GCCAATGGTGTGGTGTATAGTGACTGAATACGCCAAAGGAGGTTCAGTGAGGCAGTTTTT
GACTAGGAGACAGAACCGAGCCGTCCCTTTGAAGTTAGCTGTTAAACAGGCTTTGGATGT
TGCTAGGGGTATGGCTTATGTCCATGGACGCAACTTCATACACAGAGATCTCAAGTCAGA
TAACC

Last Updated on 06.12.2018 16:11