Sequences from confirmation amplicon


Confirmation sequences for line 722H09 and hit At2g38700 are shown.
Line ID 722H09
Locus At2g38700 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:16181279
Primers used for PCR HP77 - 8474, show primer details
   
Sequence Name 81-K033555-022-722-H09C-8409
GenBank Accession LN514014 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>81-K033555-022-722-H09C-8409
TTACAATTGAATAATCCTGCAGAAACAGGAAAGTAGCAGTTGAATTGCTGCAGGGGCTGC
TCTACTGCTTCCCTCCTAAGCCTGACACAGACATGAAGAGGTGAGATAGGAATTGTTATT
GATCTGATTGATTGATTTAATTCCTGACTAATAAAAATGGTGTTTTGGTACTTTGTATAG
TTACGTACTGGGGGATACATCGATAGTAAAAGAGGCAGGCTTGGAAGGAGAGCTTCCACA
AGGAATTAAAGACAAAATTGGAAGTCAGGATCAAAAAGGTGAAGTGAGTTATTTTATATG
CAGCAGACCTGGAAGAGGTCCTGTGGTGCTTCAAGACCAAACTCAAGCTCTTCTCCATCC
TCAAACTGGCCTCCCCAAATAACCTTNTCTATGTCA
   
Sequence Name 81-K033555-022-722-H09C-HP77
GenBank Accession LN514015 [GenBank]
Sequencing primer gene specific primer, HP77: 5'-GACATAGAAATGGTTATTTGGGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>81-K033555-022-722-H09C-HP77
TCTTGAGCACCACAGGACCTCTTCAGGTCTGCTGCATATAAAATAACTCACTTCACCTTT
TTGATCCTGACTTCCAATTTTGTCTTTAATTCCTTGTGGAAGCTCTCCTTCCAAGCCTGC
CTCTTTTACTATCGATGTATCCCCCAGTACGTAACTATACAAAGTACCAAAACACCATTT
TTATTAGTCAGGAATTAAATCAATCAATCAGATCAATAACAATTCCTATCTCACCTCTTC
ATGTCTGTGTCAGGCTTAGGAGGGAAGCAGTAGAGCAGCCCCTGCAGCAATTCAACTGCT
ACTTTCCTGTTTCTGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTAC
ATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTT
TTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGA
TAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTA
ATTCGGAAATCTTTATTTCGACGTGTCTACATTC

Last Updated on 06.12.2018 16:11