Genotyping and primer details


Genotyping and primer details for 722H09 - At2g38700
Line ID 722H09
Locus At2g38700 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:16181279 of line 722H09 Chr2:16181279
   
Insertion allele amplicon 8474 - HP77
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer HP77: 5'-GACATAGAAATGGTTATTTGGGGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:16180905
   
wt amplicon (over InsPos) 05bj - HP77
wt amplicon start Chr2:16180905
wt amplicon end Chr2:16181751
wt amplicon size 847 bp
Locus specific primer HP77: 5'-GACATAGAAATGGTTATTTGGGGA-3'
2nd locus specific primer 05bj: 5'-TCTCCTTAGGAAGTTGTCCCAGTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11