Sequences from confirmation amplicon


Confirmation sequences for line 724A02 and hit At4g09200 are shown.
Line ID 724A02
Locus At4g09200 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:5861274
Primers used for PCR CR48 - 8474, show primer details
   
Sequence Name 32-K107199-0022-724-A02C-CR48
GenBank Accession LN500608 [GenBank]
Sequencing primer gene specific primer, CR48: 5'-AAGTGACAGCTATGCAAATCAAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K107199-0022-724-A02C-CR48
ATAGACATAGCTTGTAATCACAAAGGCTTAAGTTGAAAGCAATAAACAAAAGACTTTCGA
TTTCATATTTTAACTTACAGCTAACAACAACATGTCATTAAAATTTGTAGCCGATTAAAT
TATCAAAATTAGTATGAGCAGCTCAATATTTGCCGATTTGTAGATAAATACTTGTAAATA
AAAATTTTCAAGCTCCAATATAATCTCGTATATAGTTGTGAATCAAGTTAAAGTATGATC
GCTAGTTTTAGAACATTTGAGCTTACACAATGACTCTATCAAGAGAAAAGATAAAAAGAA
AAACAGAAAAAGTCAAGATTTTATTCACGAGCATAGAGTGAGCAGGTGGCTTCAAACTGT
CTAAGCACTCGCTCAAGTGAAGCAGATTGCTGGTCTCTGGTGGCCGGATTTGTAGATAGA
ATCGCTTCACTGACTGCAGCTGCTACTATGTTCTTCTGTGTTTCTCCTAGAAGATATGCA
GCACCTGATTCTTCTATAGTTTCACACTCGAACAAAGCAGAGCAATCCTGAAATAGAGAA
AAACAAAACTATTAGTGACTCAAATATGAGAAACCTAATCTAACAGTTAGAGAATCACTA
CATACATCGAAAAGGTTTTTTAGTAGGCTTGAATCCCTAAAGTCTTTAAAATCTTGATTT
CCAAATGACTTAGCCTCTGCTATTGCTCCTTTCTGCATTGTAGTTAGTTAGAGAAACACA
CCATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGG
AGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGT
   
Sequence Name 16-K107198-0022-724-A02C-8409
GenBank Accession LN493455 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K107198-0022-724-A02C-8409
TGTTTCTCTACTAACTACAATGCAGAAAGGAGCAATAGCAGAGGCTAAGTCATTTGGAAA
TCAAGATTTTAAAGACTTTAGGGATTCAAGCCTACTAAAAAACCTTTTCGATGTATGTAG
TGATTCTCTAACTGTTAGATTAGGTTTCTCATATTTGAGTCACTAATAGTTTTGTTTTTC
TCTATTTCAGGATTGCTCTGCTTTGTTCGAGTGTGAAACTATAGAAGAATCAGGTGCTGC
ATATCTTCTAGGAGAAACACAGAAGAACATAGTAGCAGCTGCAGTCAGTGAAGCGATTCT
ATCTACAAATCCGGCCACCAGAGACCAGCAATCTGCTTCACTTGAGCGAGTGCTTAGACA
GTTTGAAGCCACCTGCTCACTCTATGCTCGTGAATAAAATCTTGACTTTTTCTGTTTTTC
TTTTTATCTTTTCTCTTGATAGAGTCATTGTGTAAGCTCAAATGTTCTAAAACTAGCGAT
CATACTTTAACTTGATTCACAACTATATACGAGATTATATTGGAGCTTGAAAATTTTTAT
TTACAAGTATTTATCTACAAATCGGCAAATATTGAGCTGCTCATACTAATTTTGATAATT
TAATCGGCTACAAATTTTAATGACATGTTGTTGTTAGCTGTAAGTTAAAATATGAAATCG
AAAGTCTTTTGTTTATTGCTTTCAACTTAAGCCTTTGTGATTACAAGCTATGTCTATTTA
TACATCTAGATTGATTTAACCTAGTGTCTTCTTGATTTG

Last Updated on 06.12.2018 16:11