Sequences from confirmation amplicon


Confirmation sequences for line 724B01 and hit At5g39730 are shown.
Line ID 724B01
Locus At5g39730 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:15901776
Primers used for PCR CR50 - 8474, show primer details
   
Sequence Name 60-K107248-0022-724-B01C-CR50
GenBank Accession LN510276 [GenBank]
Sequencing primer gene specific primer, CR50: 5'-TCCTTCAACCCAATCTACATTCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K107248-0022-724-B01C-CR50
CTGGACCCCAAACAATTCACCAAAAAAATTCTCAAAATCTAAAATTATTCAAGAATCAAG
ATCAACACACAAAGAATGATCTCAAGAAATCAAACCTTTCCATGAACTTCTCCATTCTCA
GAGGGGATAATACATGGATACAAACGTCCTTTAAGCCTAAATCTCTTACTGAAAACAACC
CAAAATCGGCATTAAACCTTAGATCTAAAAAACAGAGAGAATTATCATTCGAGATCAAGA
TCAAGATCGAGAGAGAGAGGTAACGTACAAGCCAGGAAGTGTTGCAGAGACAATCTCAGG
AATACGATTAAGCATGACATGGATGATGTCAGGCTCTTGAAAGCTACCATAGACGAAAAG
AATATGATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAAT
ATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
AGCNTTATTA
   
Sequence Name 44-K107247-0022-724-B01C-8409
GenBank Accession LN505202 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K107247-0022-724-B01C-8409
CGGACTGAGCCATCATATTCTTTTCGTCTATGGTAGCTTTCAGAGCCTGACATCATCCAT
GTCATGCTTAATCGTATTCCTGAGATTGTCTCTGCAACACTTCCTGGCTTGTACGTTACC
TCTCTCTCTCGATCTTGATCTTGATCTCGAATGATAATTCTCTCTGTTTTTTAGATCTAA
GGTTTAATGCCGATTTTGGGTTGTTTTCAGTAAGAGATTTAGGCTTAAAGGACGTTTGTA
TCCATGTATTATCCCCTCTGAGAATGGAGAAGTTCATGGAAAGGTTTGATTTCTTGAGAT
CATTCTTTGTGTGTTGATCTTGATTCTTGAATAATTTTAGATTTTGAGAATTTTTTTGGT
GAATTGTTTGGTGGTCCAGGTACTAATGGGATTGACTAATGATGAACTTGAGAATGTAGA
TTGGGTTGAAGGA
Primers used for PCR CR49 - 8474, show primer details
   
Sequence Name 61-K107248-0022-724-B01C-CR49
GenBank Accession LN510536 [GenBank]
Sequencing primer gene specific primer, CR49: 5'-AAATCCTTAAAGGTTCCACCATCT-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K107248-0022-724-B01C-CR49
CATAGTATCATACCAATTGGGTTAGTGGGTTTTGGGAAAATTTAAAGTTTTGTACTCAAG
TTGGTGTGTCTAATTATTTATTATTTACTTTATGAATTAGCATAATTAAGCAAAGAACAA
AGAATATTTTGGTCAAATATATAAAAAGATTAACATCCATCTGAAAAATCTTTTACCTAT
AGTTTTTGCAACTTGAATATTTACCTCAATAGATAAAAACTAGAGTTATTTTTTTTTTTT
TTGGCGGGAAAAAAACAAGGTTTTTTTTTAAAACCCAATTTACAAAAAAATTTTCTTATG
AGGAAAAAATTTTGAAACAAAAAAAAAAATTCCCCCGTCTTCCCCCGTTTGGGGTCCCAA
CGGTTTTTTTTTTTTTTGGGCCCCTTTAAAAACCCCAAAAAACCTTTAGGGGACCAAAAA
AAATACCAAACCCACCCCCAAAATTTTTCCATTTGAAAGGGGTTTCTTGCCGGGAAATTC
TCCTGGGACCCCCAAGGATTAGGGGGGCCAATTGGGAAAAAAAAAAAAAGAATTTCCCAT
TTTTTTTCCATTTAAAAAAGGAAAATGCCCCCGCCTTTATAAAAAAAA
   
Sequence Name 45-K107247-0022-724-B01C-oriL
GenBank Accession LN505547 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K107247-0022-724-B01C-oriL
TTACATTGAATATATCTTGGGATTGGTTATGTTATTTTCTCTGATCCTCTAGAGCTTTTT
GAGGTTTTATAACGTGACCAAAAAAAAGAATAACACGTAGGAACACGAAACGGTGAACGA
CGTGGAATTATGTTCTTTAGTTACAAATTTTTGTCATCATAGTAGATTATTTTGCTAACT
TGGCTTTGATAAATAACTCTAGTTTTTTGCCGACAAAAAAAAAAAAAAAAACCTCTGTTT
TTTTTTTTTGGGGGAAAATTTCAAGTTGGAAAAAATTTTGGGAAAAAATTTTTCCAAAGG
GGGGTAATCTTTTTTATTTTTTGACCAAAAATTTCTTTGGTTTTTGGTTAATTTGGCCAA
TTTCAAAAGGAAAAAAAAAAAAATTTAAACCCCCCACTTGGGGAACAAATTTTAAATTTT
CCCAAAACCCCCTAACCCAATTGGGTTTGGTTTCCAATTGGGTTTAGCCCTTTAAGGGGG
GGAACCTTTAAGGGTTTA

Last Updated on 06.12.2018 16:11