Sequences from confirmation amplicon


Confirmation sequences for line 724C11 and hit At3g12400 are shown.
Line ID 724C11
Locus At3g12400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:3944743
Primers used for PCR CR51 - 8474, show primer details
   
Sequence Name 60-K106210-0022-724-C11C-CR51
GenBank Accession LN510267 [GenBank]
Sequencing primer gene specific primer, CR51: 5'-AGGTTTTCAAGAGAAATGCGATTA-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K106210-0022-724-C11C-CR51
CGATTTGGTTCGATGAGGAGAGCCGAGAAGCTGAAGCAGAGGAGCTGCTGAGCTTGCAAG
CTGGGCTGAAGAGAAGAGAGGATGAGCTTAATATAGGGTTGAAAGAGATGGTTGAGGAGA
AAGAAACACTTGAACAACAATTACAGATTATCTCCATGAACACTGATATTCTAGACTCGT
GGGTTAGAGAGAACCAAGGCAAAACCAAGAATTTAGTTGATTTGGATGTGGATAATGCTT
TTGAATGTGGTGACACACTCTCTAAGCAGATGTTAGAGTGTACTGCTTTGGATTTAGCCA
TTGAAGATGCTATTTATTCCTTGGATAAGTCGTTTCAAGATGCTTCAGGATATATTCAAT
TGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 76-K106211-0022-724-C11C-8409
GenBank Accession LN513404 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>76-K106211-0022-724-C11C-8409
ATTTACAATTGAATAATCCTGAAGCATCTTGAAACGACTTATCCAAGGAATAAATAGCAT
CTTCAATGGCTAAATCCAAAGCAGTACACTCTAACATCTGCTTAGAGAGTGTGTCACCAC
ATTCAAAAGCATTATCCACATCCAAATCAACTAAATTCTTGGTTTTGCCTTGGTTCTCTC
TAACCCACGAGTCTAGAATATCAGTGTTCATGGAGATAATCTGTAATTGTTGTTCAAGTG
TTTCTTTCTCCTCAACCATCTCTTTCAACCCTATATTAAGCTCATCCTCTCTTCTCTTCA
GCCCAGCTTGCAAGCTCAGCAGCTCCTCTGCTTCAGCTTCTCTGGCTCTCCTCATCGAAA
CCAAATCGCTATGAACCATCTCCACCATCTTATTAATCGCATTTCTCTTGAAAAC

Last Updated on 06.12.2018 16:11