Sequences from confirmation amplicon


Confirmation sequences for line 724G08 and hit At1g08450 are shown.
Line ID 724G08
Locus At1g08450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:2670872
Primers used for PCR SP11 - 8474, show primer details
   
Sequence Name 52-K103548-0022-724-G08C-SP11
GenBank Accession LN507787 [GenBank]
Sequencing primer gene specific primer, SP11: 5'-CAGGACGCAGAATAAATGTGTAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K103548-0022-724-G08C-SP11
TGCAATCCTTTTTGATTGGATAATTCTGTCCCTGATATGAAACTATAACATGAAGCTTCT
TCGTCTGTGTTCCGCATATATCTGGTCCAAACATTAGACTGCATAGAAAAACTAATGAGA
ATCCCCAAACACAACAACACCATGTGACGACTTTGTTGTAAAAAACATGGACATGTAATC
ATTTATCAAAGTTCACTGGATTAACTAAATCAACGCAACTTGACTTAGATTGCAATATAA
CATTCTATCAACTGATGTTCATTGAAAAAGCATTAATGTATGATTACCTGTATGGAGTAT
CACCACCAAACTGCTTCTGGTTAACATAGCCAGACAGGAGCTTAATGTAAGCACCACCAC
ATTCAATGTCTTGTTCAATCTTGACTGAGTACTGGACAACAAGAGTCCGGTTTTTGTTGC
TAAACTCAGGAATCTTTGCAGAAATCGCATAATGCTTGGCATCATTATAAGTCTGAATAC
CTAAACACATCAAGAAAATCAAAAAAGAAAGAAAAAGATACTAAGTTAAACCAAATAAAG
ATTCTATATTCCTAGAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTA
TGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATG
TAGATGTCCGCA
   
Sequence Name 36-K103549-0022-724-G08C-8409
GenBank Accession LN502195 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K103549-0022-724-G08C-8409
GCCATTTCAGGAATATAGAATCTTTATTTGGTTAACTTAGTATCTTTTTCTTTCTTTTTT
GATTTTCTTGATGTGTTTAGGTATTCAGACTTATAATGATGCCAAGCATTATGCGATTTC
TGCAAAGATTCCTGAGTTTAGCAACAAAAACCGGACTCTTGTTGTCCAGTACTCAGTCAA
GATTGAACAAGACATTGAATGTGGTGGTGCTTACATTAAGCTCCTGTCTGGCTATGTTAA
CCAGAAGCAGTTTGGTGGTGATACTCCATACAGGTAATCATACATTAATGCTTTTTCAAT
GAACATCAGTTGATAGAATGTTATATTGCAATCTAAGTCAAGTTGCGTTGATTTAGTTAA
TCCAGTGAACTTTGATAAATGATTACATGTCCATGTTTTTTACAACAAAGTCGTCACATG
GTGTTGTTGTGTTTGGGGATTCTCATTAGTTTTTCTATGCAGTCTAATGTTTGGACCAGA
TATATGCGGAACACAGACGAAGAAGCTTCATGTTATAGTTTCATATCAGGGACAGAATTA
TCCAATCAAAAAGGATTTGCAATGTGAAACAGACAAATTAAATCATTTCTACACATTTAT
CTGNCGTCCT

Last Updated on 06.12.2018 16:11