Genotyping and primer details


Genotyping and primer details for 724G08 - At1g08450
Line ID 724G08
Locus At1g08450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:2670872 of line 724G08 Chr1:2670872
   
Insertion allele amplicon 8474 - SP11
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer SP11: 5'-CAGGACGCAGAATAAATGTGTAGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:2670269
   
wt amplicon (over InsPos) 08bg - SP11
wt amplicon start Chr1:2670269
wt amplicon end Chr1:2671147
wt amplicon size 879 bp
Locus specific primer SP11: 5'-CAGGACGCAGAATAAATGTGTAGA-3'
2nd locus specific primer 08bg: 5'-TGATATGTTCTTGTTAGCTGCCAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11